Histochemistry

, Volume 85, Issue 5, pp 439–440 | Cite as

Autofluorescence and induced fluorescence in Epon embedded tissue sections

  • P. del Castillo
  • M. L. Molero
  • J. M. Ferrer
  • J. C. Stockert
Short Communications

Summary

The autofluorescence of tissue components in Epon semithin sections depends on the exciting wavelength, section thickness, and the structure under observation. The degree of autofluorescence emission of a given component affects the fluorescence reaction induced by fluorochromes, a feature which must be taken into account when fluorescence microscopy is applied to the study of Epon sections.

Keywords

Public Health Fluorescence Microscopy Tissue Section Section Thickness Tissue Component 

References

  1. Biebl R, Germ H (1967) Praktikum der Pflanzenanatomie. 2. Aufl. Springer, Wien New York, pp 112–113Google Scholar
  2. Dietrich R (1979) Immunfluoreszenz. In: Witte S, Ruch F (eds) Moderne Untersuchungsmethoden in der Zytologie, 2. Aufl. Gerhard Witzstrock, Baden-Baden Köln New York, pp 70–81Google Scholar
  3. Horobin RW (1982) Staining plastic sections: a review of problems, explanations and possible solutions. J Microsc 131:173–186Google Scholar
  4. Kasten FH (1981) Methods for fluorescence microscopy In: Clark G (ed) Staining procedures. Williams & Wilkins, Baltimore London, pp 39–103Google Scholar
  5. Litwin JA (1985) Light microscopic histochemistry on plastic sections. Prog Histochem Cytochem 16:1–84Google Scholar
  6. Molero ML, Hazen MJ, Stockert JC (1985) Observations on the orcein fluorescence. Acta Histochem 76:77–79Google Scholar
  7. Nairn RC (1976) Fluorescent protein tracing. 4th edn. Churchill Livingstone, EdinburghGoogle Scholar
  8. Pearse AGE (1972) Histochemistry. Theoretical and applied, vol 2. 3rd. edn. J and A Churchill, London, pp 1171–1206Google Scholar
  9. Stockert JC, Ferrer JM, González-Garrigues M (1984) Autofluorescence of eosinophil leukocyte granules. Biol Zbl 103:543–546Google Scholar
  10. Thompson EB (1966) Selected histochemical and histopathological methods. CC Thomas, Springfield, Illinois, pp 209–222Google Scholar
  11. Udenfriend S (1962) Fluorescence assay in biology and medicine, vol 1. Academic Press, New York London, pp 201–214Google Scholar
  12. Wittekind D (1979) Fluoreszenzmikroskopie. In: Witte S, Ruch F (eds) Moderne Untersuchungsmethoden in der Zytologie. 2. Aufl. Gerhard Witzstrock, Baden-Baden Köln New York, pp 36–69Google Scholar

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • P. del Castillo
    • 1
    • 2
  • M. L. Molero
    • 1
    • 2
  • J. M. Ferrer
    • 1
    • 2
  • J. C. Stockert
    • 1
    • 2
  1. 1.Departamento de Citología, Facultad de CienciasUniversidad Autónoma de MadridMadridSpain
  2. 2.Departamento de Morfología Microscópica, Facultad de BiologíaUniversidad de ValenciaSpain

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