Abstract
UPD-glucose transferases are found in the cytosolic and microsomal fractions of the grain aphidSitobion avenae F. Gel filtration and SDSPAGE revealed that the microsomal fraction contained several forms of the enzyme. The molecular weights of the three most active fractions might be 68,000, 66,000, and 36,500. There was a negative correlation between the enzymes' activity in extracts of aphids and the concentration of DIMBOAaglucone in the winter wheat variety fed on by the aphid. A strong inhibition of the activity of the UPD-glucose transferases was observedin vitro at a concentration of DIMBOA as low as 0.01 mM. There was a greater activity of the enzymes in aphids fed on seedlings of susceptible than on moderately resistant wheat cultivars. Prolonged feeding on resistant cultivars resulted in a further reduction in the activity of the aphid's enzymes. The significance for cereal aphids of the role of their UDP-glucose tranferases in the detoxification of plant allelochemicals and adaptation to resistant varieties of cereals is discussed.
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References
Ahmad, S., Brattsten, L.B., Mullin, C.A., andYu, S.J. 1986. Enzymes involved in the metabolism of plant allelochemicals, pp. 73–152,in L.B. Brattsten and S. Ahmad (eds.). Molecular Aspects of Insect-Plant Associations. Plenum Press, New York and London.
Argando¯na, V.H., Luza, J.G., Niemeyer, H.M., andCorcuera, L.J. 1980. Role of hydroxamic acids in the resistance of cereals to aphids.Phytochemistry 19:1665–1668.
Argando¯na, V.H., Corcuera, L.J., Niemeyer, H.M., andCampbell, B.C. 1983. Toxicity and feeding deterrancy of hydroxamic acids from Graminae in synthetic diets against the greenbugSchizaphis graminum.Entomol. Exp. Appl. 34: 134–138.
Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Anal. Biochem. 72:148–254.
Berenbaum, M.R. 1985. Synergistic action among allelochemicals in crop plants, No. 75,in Book of Abstracts, 190th ACS National Meeting, Chicago, Sept. 8–13.
Chipoulet, J.M., andChararas, C. 1985. Survey and electrophoretical separation of glucosidases ofRhagium inguistor (Coleoptera: Cerambycidae) larvae.Comp. Biochem. Physiol. 808:241–246.
Cuevas, L., Niemeyer, H.M., andPerez, F.J. 1990. Reaction of DIMBOA, a resistance factor from cereals with α-chymotripsin.Phytochemistry 29:1429–1432.
Dauterman, W.C. 1985. Insect metabolism: Extramicrosomal, pp. 713–733,in G.A. Kerkut and Gilbert, L.I. (eds.). Comprehensive Insect Physiology, Biochemistry and Pharmacology, Vol. 12. Pergamon Press, New York.
Dixon, A.F.G. 1987. Cereal aphids as an applied problem.Agr. Zool. Rev. 2:1–57.
Dorough, H.W. 1979. Metabolism of insecticides by conjugation mechanisms.Pharmacol. Ther. 4:433–471.
Dreyer, D.L., andJones, K.C. 1981. Feeding deterency of flavonoids and related phenolics towardsSchizaphis graminum andMyzus persicae: Aphid feeding deterrents in wheat.Phytochemistry 20:2489–2493.
Fishbein, W.N. 1982. Hydroxamic acids as urease inhibitors for medical and veterinary use, pp. 94–103,in H. Kehl (ed.). Chemistry and Biology of Hydroxamic Acids. Karger, Basel.
Harborne, J.B. 1988. Phenolic compounds, pp. 37–39,in J.B. Harborne (ed.). Phytochemical Methods, 2nd ed. Chapman and Hall, London, New York.
Katagiri, Ch. 1979.α-Glucosidase in the midgut of the American cockroach,Periplaneta americana.Insect Biochem. 9:205–209.
Kent, P.W., andBrunet, P.C.J. 1959. The occurrence of protocatechuic acid and its 4-0-β-D-glucoside inBlatta andPeriplaneta.Tetrahedron 7:252–256.
Kjoller, L.I., Sjoberg, B.M., andThelander, L. 1982. Hydroxamic acids and other hydroxylamine derivatives as inhibitors of DNA synthesis, pp. 83–93,in H. Kehl (ed.). Chemistry and Biology of Hydroxamic Acids, Karger, Basel.
Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head bacteriophage T.Nature (London)227:680–685.
Leszczynski, B. 1987. Winter wheat resistance in the grain aphidSitobion avenae (Fabr.) (Homoptera, Aphididae).Insect Sci. Appl. 8:251–254.
Leszczynski, B., andDixon, A.F.G. 1990. Resistance of cereals to aphids: Interaction between hydroxamic acids and the aphidSitobion avenae (Homoptera: Aphididae).Ann. Appl. Biol. 117:21–30.
Leszczynski, B., andDixon, A.F.G. 1992. Resistance of cereals to aphids: The interaction between hydroxamic acids and the glutathione S-transferases in the grain aphidSitobion avanae (Homoptera: Aphididae).J. Appl. Entomol. 113:61–67.
Leszczynski, B., Warchol, J., andNiraz, S. 1985. The influence of phenolic compounds on preference of winter wheat cultivars by cereal aphids.Insect Sci. Appl. 6:157–158.
Lindroth, R.L. 1988. Hydrolysis of phenolic glucosides by midgutβ-glucosidases inPapilio glaucus subspecies.Insect Biochem. 18:789–792.
Lindroth, R.L. 1989. Host plant alteration of detoxyfication activity inPapilio glaucus glaucus.Entomol. Exp. Appl. 50:29–35.
Mehendale, H.M., andDorough, H.W. 1972. In vitro glucosylation of 1-naphthol by insects.J. Insect Physiol. 18:981–987.
Morello, A., andRepetto, Y. 1979. UDP-glucosyltransferase activity of housefly microsomal fraction.Biochem. J. 177:809–812.
Morello, A., Bleeker, W., andAgosin, M. 1971. Cytochrome P-450 and hydroxylating activity of microsomal preparations from houseflies.Biochem. J. 124:199–205.
Niemeyer, H.M. 1988. Hydroxamic acids (4-hydroxy-1,4-benzoxazin-3-ones) defense chemicals in the Graminae.Phytochemistry 27:3349–3358.
Niemeyer, H.M., Pesel, E., Copaja, S.V., Bravo, H.R., Franke, S., andFrancke, W. 1989a. Changes in hydroxamic acid levels in wheat plants induced by aphid feeding.Phytochemistry 28:447–449.
Niemeyer, H.M., Pesel, E., Franke, S., andFrancke, W. 1989b. Ingestion of the benzoxazolinone DIMBOA from wheat plants by aphids.Phytochemistry 28:2307–2310.
Perez, F.J., andNiemeyer, H.M. 1989. Reaction of DIMBOA a resistance factor from cereals with papain.Phytochemistry 28:1597–1600.
Pridham, J.B. 1960. The formation and possible function of phenolic glucosides, pp. 9–15,in J.B. Pridham (ed.). Proceedings of a Plant Phenolics Group Symposium, Bristol, April. Pergamon Press, Oxford, London, New York, Paris.
Queirolo, C.B., Andreo, C.S., Niemeyer, H.M., andCorcuera, L.J. 1983. Inhibition of ATPase from chloroplasts by a hydroxamic acids from the Graminae.Phytochemistry 22:2455–2458.
Smith, J.M. 1968. The comparative metabolism of xenobiotics.Adv. Comp. Physiol. Biochem. 3:173–232.
Smith, J.M., andTurbert, H.B. 1961. Enzymic glucoside synthesis in locusts.Nature 189:600.
Teas, H.J. 1967. Cycasin synthesis inSeirarctia echo (Lepidoptera) larvae fed methylazaxymethanol.Biochem. Biophys. Res. Comm. 26:689–690.
Todd, G.W., Getahun, A., andCress, D.C. 1971. Resistance in barley to the greenbugSchizaphis graminum. I. Toxicity of phenolic and flavonoid compounds and related substances.Ann. Entomol. Soc. Am. 64:708–722.
Trivelloni, J.C. 1964. Estudio sobre la farmacion deβ-glucosidos en la langosta (Schistocerca cancelata).Enzymologia 26:329–339.
Wahlross, O., andVirtanen, A.I. 1959. Precursors of 6-methoxy-benzoxazolinone in maize and wheat plants, their isolation and some of their properties.Acta Chem. Scand. 13:1906–1908.
Weber, K., andOsborn, M. 1968. The reliability of molecular weight determination by dodecyl sulphate-polyacrylamide gel electrophoresis.J. Biol. Chem. 244:4406–4412.
Yang, R.H.S. 1976. Enzymatic conjugation and insecticide metabolism, pp. 175–225,in C.F. Wilkinson (ed.). Insecticide Biochemistry and Physiology. Plenum, New York.
Zu¯niga, G.E., Salgado, M.S., andCorcuera, L.J. 1985. Role of an indole alkaloid in the resistance of barley seedlings to aphids.Phytochemistry 24:945–947.
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Leszczynski, B., Matok, H. & Dixon, A.F.G. Resistance of cereals to aphids: The interaction between hydroxamic acids and UDP-glucose transferases in the aphidSitobion avenue (Homoptera: Aphididae). J Chem Ecol 18, 1189–1200 (1992). https://doi.org/10.1007/BF00980073
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DOI: https://doi.org/10.1007/BF00980073