Neurochemical Research

, Volume 12, Issue 2, pp 173–178 | Cite as

In vivo release of endogenous dopamine, 5-hydroxytryptamine and some of their metabolites from rat caudate nucleus by phenylethylamine

  • B. A. Bailey
  • S. R. Philips
  • A. A. Boulton
Original Articles

Abstract

The in vivo release of endogenous 3,4-dihydroxyphenylethylamine (DA) and its metabolites, 3,4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA) and 3-methoxytyramine (3-MT), and of 5-hydroxytryptamine (5-HT) and its metabolite, 5-hydroxyindoleacetic acid (5-HIAA), has been measured in the caudate nucleus of the anesthetized rat. A push-pull cannula was implanted into the brain, and the tissue perfused with artificial CSF or artificial CSF containing 5×10−4 M phenylethylamine. The perfusate was collected and analyzed for DA, 5-HT and their metabolites by high performance liquid chromatography with electrochemical detection (HPLC-ECD). DA was released by phenylethylamine at rates significantly greater than its basal rate. 3-MT and 5-HT were undetectable in perfusates collected under basal conditions, but could be detected readlly during phenylethylamine stimulation. DOPAC, HVA and 5-HIAA concentrations were not significantly affected by phenylethylamine. The results suggest (1) that phenylethylamine may exert its behavioural effects through increased release of both DA and 5-HT, and (2) that in vivo measurements of the acid metabolites alone may not be indicative of the release of the amines.

Key Words

Dopamine release 5-HT release phenylethylamine push-pull perfusion 

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Copyright information

© Plenum Publishing Corporation 1987

Authors and Affiliations

  • B. A. Bailey
    • 1
  • S. R. Philips
    • 1
  • A. A. Boulton
    • 1
  1. 1.Psychiatric Research Division, Saskatchewan Health, Cancer and Medical Research BuildingUniversity of SaskatchewanSaskatoonCanada

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