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Salivary glands and preputial glands of males as source of estrus-stimulating pheromone in female mice

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Abstract

Male mice produce pheromones that induce estrus in adult female mice. The synthesis of these chemosignals is controlled by testosterone. Male tissues abundant in testosterone-binding receptors were tested for their ability to stimulate estrus in grouped females. The percentage of females that showed estrus during two days following exposure to male tissues was used as the indicator of biological activity of tested samples. Homogenates of salivary glands and preputial glands induced estrus; other tested organs (liver, testes, coagulation and vesicular glands or kidneys) did not stimulate estrus in female mice. These results indicate that estrus-stimulating pheromone has multiple sources.

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References

  • Bronson, F.H. 1971. Rodent pheromones.Biol. Reprod. 4:344–357.

    PubMed  Google Scholar 

  • Bronson, F.H., andCaroom, D. 1971. Preputial gland of the male mouse: attractant function.J. Reprod. Fertil. 25:279–282.

    PubMed  Google Scholar 

  • Bronson, F.H., andMaruniak, J.A. 1975. Male-induced puberty in female mice: Evidence for a synergistic action of social cues.Biol. Reprod. 13:94–98.

    PubMed  Google Scholar 

  • Bronson, F.H., andWhitten, W.K. 1968. Oestrus-accelerating pheromones of mice: Assay, androgen dependency and presence in bladder urine.J. Reprod. Fertil. 15:131–134.

    PubMed  Google Scholar 

  • Bruce, H.M. 1959. An exteroreceptive block to pregnancy in the mouse.Nature 184:105.

    PubMed  Google Scholar 

  • Chipman, R.K., andAlbrecht, E.D. 1974. The relationship of the male preputial gland to the acceleration of oestrus in the laboratory mouse.J. Reprod. Fertil. 38:91–96.

    PubMed  Google Scholar 

  • Colby, D.R., andVandenbergh, J.G. 1974. Regulatory effects of urinary pheromones on puberty in the mouse.Biol. Reprod. 11:268–279.

    PubMed  Google Scholar 

  • Dominic, C.J. 1965. The origin of the pheromones causing pregnancy block in mice.J. Reprod. Fertil. 10:469–472.

    PubMed  Google Scholar 

  • Drickamer, L.C., andMurphy, R.X. 1978. Female mouse maturation: Effects of excreted and bladder urine from juvenile and adult males.Dev. Psychobiol. 11:63–72.

    PubMed  Google Scholar 

  • Finlayson, J.S., Asofsky, R., Potter, M., andRunner, C.C. 1965. Major urinary protein complex of normal mice: Origin,Science 149:981–982.

    PubMed  Google Scholar 

  • Gaunt, S.L. 1968. Studies on the preputial gland as a source of a reproductive pheromone in the laboratory mouse (Mus musculus). Ph.D. thesis. Burlington, University of Vermont.

    Google Scholar 

  • Held, W.A. andGallagher, J.F. 1985. Ratα 2u-globulin mRNA expression in the preputial gland.Biochem. Genet. 23:281–290.

    PubMed  Google Scholar 

  • Hoppe, P.C. 1975. Genetic and endocrine studies of the pregnancy blocking pheromones of mice.J. Reprod. Fertil. 45:109–115.

    PubMed  Google Scholar 

  • Marchlewska-Koj, A. 1977. Pregnancy block elicited by urinary proteins of male mice.Biol. Reprod. 17:729–732.

    PubMed  Google Scholar 

  • Marchlewska-Koj, A., andBiały, E. 1978. Modification of the oestrus cycle by urinary proteins of male mice.Folia Biol. (Crac.) 26:311–314.

    Google Scholar 

  • Marchlewska-Koj, A., andKruczek, M. 1986. Acceleration and suppression of the oestrous cycle in mice by pheromones: The light effect.Folia Biol. (Crac.) 34:221–226.

    Google Scholar 

  • Milligan, S.R., 1974. Social environment and ovulation in the vole,Microtus agrestis.J. Reprod. Fertil. 41:35–47.

    PubMed  Google Scholar 

  • Milligan, S.R. 1975. Further observations on the influence of the social environment on ovulation in the vole,Microtus agrestis.J. Reprod. Fertil. 44:543–544.

    PubMed  Google Scholar 

  • Mugford, R.A. andNowell, N.W. 1971. The preputial glands as a source of aggression-promoting odors in mice.Physiol. Behav. 6:247–249.

    PubMed  Google Scholar 

  • Ninomiya, K., andKimura, T. 1988. Male odors that influence the preference of female mice: Roles of urinary and preputial factors.Physiol. Behav. 44:791–795.

    PubMed  Google Scholar 

  • Shaw, P.H., Held, W.A., andHastie, N.D. 1983. The gene family for major urinary proteins: Expression in several secretory tissues of the mouse.Cell 32:755–761.

    PubMed  Google Scholar 

  • Szoka, P., Gallagher, J, andHeld, W. 1980. In vitro synthesis and characterization of precursors to the mouse major urinary proteins.J. Biol. Chem. 255:1367–1373.

    PubMed  Google Scholar 

  • Vandenbergh, J.G. 1967. Effect of the presence of a male on the social maturation of female mice.Endocrinology 81:345–359.

    PubMed  Google Scholar 

  • Vandenbergh, J.G. 1969. Male odor accelerates female sexual maturation in mice.Endocrinology 84:658–660.

    PubMed  Google Scholar 

  • Vandenbergh, J.G., Whitsett, J.M., andLombardii, J.R. 1975 Partial isolation of pheromone accelerating puberty in female mice.J. Reprod. Fertil. 43:515–523.

    PubMed  Google Scholar 

  • Whitten, W.K. 1956. Modification of the oestrous cycle of the mouse by external stimuli associated with the male.J. Endocrinol. 13:399–404.

    PubMed  Google Scholar 

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Marchlewska-Koj, A., Pochroń, E. & Śliwowska, A. Salivary glands and preputial glands of males as source of estrus-stimulating pheromone in female mice. J Chem Ecol 16, 2817–2822 (1990). https://doi.org/10.1007/BF00979475

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  • DOI: https://doi.org/10.1007/BF00979475

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