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Involvement of phospholipase A2 and arachidonic acid in the depolarization-evoked accumulation of Ca2+ in hippocampal mossy fiber nerve endings

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Abstract

Depolarization-evoked increases in intraterminal free Ca2+ are required for the induction of neurotransmitter release from nerve terminals. Although the mechanisms that regulate the voltage-induced accumulation of presynaptic Ca2+ remain obscure, there is evidence that the phospholipase-dependent accumulation of arachidonic acid, or its metabolites, may be involved. Therefore, fura-2 loaded hippocampal mossy fiber nerve endings were used to investigate the relationships between membrane depolarization, lipid metabolism and presynaptic Ca2+ availability. It was observed that depolarization of the nerve terminals with KCl induced an increase in intraterminal free calcium that was inhibited more than 90% by a combination of voltage-sensitive Ca2+ channel blockers. In addition, the K+-dependent effects on Ca2+ concentrations were attenuated in the presence of phospholipase A2 inhibitors, but were mimicked by the phospholipase A2 activator melittin and exogenous arachidonic acid. Both the melittin- and arachidonic acid-induced increases in presynaptic Ca2+ were reduced by voltage-sensitive Ca2+ channel blockers. The stimulatory effects of arachidonic acid appeared to be independent of its further metabolism to prostaglandins. In fact, inhibition of either cyclooxygenase or lipoxygenase pathways resulted in a potentiation of the depolarization-evoked increase in intraterminal free Ca2+. From these results, we propose that some portion of the depolarization-evoked increase in intraterminal free calcium depends on the activation of phospholipase A2 and the subsequent accumulation of unesterified arachidonic acid.

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References

  1. Lazarewicz, J. W., Leu, V., Sun, G. Y., and Sun, A. Y. 1983. Arachidonic acid release from K+-evoked depolarization of brain synaptosomes. Neurochem. Int. 5:471–478.

    Google Scholar 

  2. Frye, R. A., and Holz, R. W. 1984. The relationship between arachidonic acid release and catecholamine secretion from cultured bovine adrenal chromaffin cells. J. Neurochem. 43:146–150.

    Google Scholar 

  3. Asakura, T., and Matsuda, M. 1984. Efflux of gamma-amino-butyric acid from and appearance of free arachidonic acid inside synaptosomes. Biochim. Biophys. Acta 773:301–307.

    Google Scholar 

  4. Dorman, R. V., Schwartz, M. A., and Terrian, D. M. 1986. Prostaglandin involvement in the evoked release of D-aspartate from cerebellar mossy fiber terminals. Brain Res. Bull. 17:243–248.

    Google Scholar 

  5. Verhage, M., Besselsen, E., Lopes da Silva, F., and Ghijsen, W. 1988. Evaluation of the Ca2+ concentration in purified nerve terminals: Relationship between Ca2+ homeostasis and synaptosomal preparation. J. Neurochem. 51:1667–1674.

    Google Scholar 

  6. Verhage, M., Besselsen, E., Lopes da Silva, F., and Ghijsen, W. 1989. Ca2+-dependent regulation of presynaptic stimulus-secretion coupling. J. Neurochem. 53:1188–1194.

    Google Scholar 

  7. Bradford, P. G., Marinetti, G. V., and Abood, L. G. 1983. Stimulation of phospholipase A2 and secretion of catecholamines from brain synaptosomes by potassium and A23187. J. Neurochem. 41:1684–1693.

    Google Scholar 

  8. Lynch, M. A., and Voss, K. L. 1991. Presynaptic changes in long-term potentiation: elevated synaptosomal calcium concentration and basal phosphoinositide turnover in dentate gyrus. J. Neurochem. 56:113–118.

    Google Scholar 

  9. Kandasamy, S. G., and Hunt, W. A. 1990. Arachidonic acid and prostaglandins enhance potassium-stimulated calcium influx into rat brain synaptosomes. Neuropharmacol. 29:825–829.

    Google Scholar 

  10. Freeman, E. J., Terrian, D. M. and Dorman, R. V. 1990. Presynaptic facilitation of glutamate release from isolated hippocampal mossy fiber nerve endings by arachidonic acid. Neurochem. Res. 15:749–756.

    Google Scholar 

  11. Carlson, R. O., and Levitan, I. B. 1990. Regulation of intracellular free arachidonic acid in Aplysia nervous system. J. Memb. Biol. 116:249–260.

    Google Scholar 

  12. Garcia-Martin, E., Gonzalez-Cabanillas, S., and Gutierrez-Merino, C. 1990. Modulation of calcium fluxes across synaptosmal plasma membrane by local anesthetics. J. Neurochem. 55:370–378.

    Google Scholar 

  13. Bradford, P. G., and Marinetti, G. V. 1982. Effects of local anesthetics on phospholipid topology and dopamine uptake and release in rat brain synaptosomes. J. Memb. Biol. 67:211–218.

    Google Scholar 

  14. Sun, A. Y. 1985. Involvement of phospholipase A2 in norepinephrine release from synaptosomes isolated from rat cerebral cortex. Neurochem. Int. 7:1055–1060.

    Google Scholar 

  15. Chan, K. M., and Turk, J. 1987. Mechanism of arachidonic acid-induced Ca2+ mobilization from rat liver microsomes. Biochim. Biophys. Acta 928:186–193.

    Google Scholar 

  16. Rustenbeck, I., and Lenzen, S. 1989. Regulation of transmembrane ion transport by reaction products of phospholipase A2-Effects of arachidonic acid and other fatty acids on mitochondrial Ca2+ transport. Biochim. Biophys. Acta 982:147–155.

    Google Scholar 

  17. Freeman, E. J., Damron, D. S., Terrian, D. M., and Dorman, R. V. 1991. 12-Lipoxygenase products attenuate the glutamate release and Ca2+ accumulation evoked by depolarization of hippocampal mossy fiber nerve endings. J. Neurochem. 56:1079–1082.

    Google Scholar 

  18. Billah, M. M., Bryant, R. W., and Siegel, M. I. 1985. Lipoxygenase products of arachidonic acid modulate biosynthesis of platelet activating factor (1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) by human neutrophils via phospholipase A2. J. Biol. Chem. 260:6899–6906.

    Google Scholar 

  19. Robison, T. W., Sevanian, A., and Forman, H. J. 1990. Inhibition of arachidonic acid release by nordihydroguaiaretic acid and its antioxidant action in rat alveolar macrophages and Chinese hamster lung fibroblasts. Toxicol. Applied Pharmacol. 105:113–122.

    Google Scholar 

  20. Terrian, D. M., Johnston, D., Claiborne, B. J., Ansah-Yiadom, R., Strittmatter, W. J., and Rea, M. A. 1988. Glutamate and dynorphin release from a subcellular fraction enriched in hippocampal mossy fiber synaptosomes. Brain Res. Bull. 21:343–351.

    Google Scholar 

  21. Terrian, D. M., Damron, D. S., Dorman, R. V. and Gannon, R. L. 1989. Effects of calcium antagonists on the evoked release of dynorphin A(1–8) and availability of intraterminal calcium in rat hippocampal mossy fiber synaptosomes. Neurosci. Letts. 106:322–327.

    Google Scholar 

  22. Layne, E. 1957. Spectrophotometric and turbidimetric methods for measuring protein. Pages 447–454, in Colowick, S. P., and Kaplan, N. P. (eds.), Methods in Enzymology, Vol. III. Academic Press, New York.

    Google Scholar 

  23. Grynkiewicz, G., Poenie, M., and Tsien, Y. 1985. A new generation of calcium indicators with greatly improved fluorescence properties. J. Biol. Chem. 260:3440–3450.

    Google Scholar 

  24. Separovic, D., and Dorman, R. V. 1993. Prostaglandin F2alpha synthesis in the hippocampal mossy fiber synaptosomal preparation: I. Dependence on arachidonic acid, phospholipase A2, calcium availability and membrane depolarization. Prostaglan., Leukot. Essent. Fatty Acids 48:127–137.

    Google Scholar 

  25. Terrian, D. M., Dorman, R. V., Damron, D. S. and Gannon, R. L. 1991. Displacement of endogenous glutamate with D-aspartate: an effective strategy for reducing the calcium-independent component of glutamate release from synaptosomes. Neurochem. Res. 16:35–41.

    Google Scholar 

  26. Cass, W. A., Larson, G., Fitzpatrick, F. A., and Zahniser, N. R. 1991. Inhibitors of arachidonic acid metabolism: Effects on rat striatal dopamine release and uptake. J. Pharmacol. Exp. Ther. 257:990–997.

    Google Scholar 

  27. Conricode, K. M., and Ochs, R. S. 1989. Mechanism for the inhibition and stimulatory actions of proteins on the activity of phospholipase A2. Biochim. Biophys. Acta 1003:36–43.

    Google Scholar 

  28. Linden, J. D., and Routtenberg, A. 1989. Cis-fatty acids, which activate protein kinase C, attenuate Na+ and Ca2+ currents in mouse neuroblastoma cells. J. Physiol. 419:95–119.

    Google Scholar 

  29. Terrian, D. M., Ways, D. K., and Gannon, R. L. 1991. A presynaptic role for protein kinase C in hippocampal mossy fiber synaptic transmission. Hippocampus 1:303–314.

    Google Scholar 

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Authors and Affiliations

  1. Department of Biological Sciences, Kent State University, 44242, Kent, Ohio

    Derek S. Damron & Robert V. Dorman

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  1. Derek S. Damron
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  2. Robert V. Dorman
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Damron, D.S., Dorman, R.V. Involvement of phospholipase A2 and arachidonic acid in the depolarization-evoked accumulation of Ca2+ in hippocampal mossy fiber nerve endings. Neurochem Res 18, 1231–1237 (1993). https://doi.org/10.1007/BF00975040

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