On the activity of γ-aminobutyric acid and glutamate transporters in chick embryonic neurons and rat synaptosomes
- 15 Downloads
The uptake of radioactive γ-aminobutyric acid (GABA) andd-aspartate and the effect of SKF 89976-A, a non-substrate inhibitor of the GABA transporter, on this uptake have been investigated. Neuronal cultures from eight-day-old chick embryos grown for three or six days in vitro, were used as a model. For comparison, we also used the P2-fraction from rat. Neuronal cultures grown for three and six days expressed high-affinity uptake systems for [3H]GABA and ford-[3H]aspartate with an increasing Vmax during this period. The lipophilic non-substrate GABA uptake inhibitor, SKF 89976-A, inhibited transporter mediated uptake of GABA both in cell cultures from chicken, and in P2-fractions from rat. The results also showed that SKF 89976-A was a poor inhibitor of the uptake ofd-aspartate. We found no non-saturable uptake ofd-aspartate.
Key WordsGABA d-aspartate glutamate primary neuronal culture rat synaptosomes SKF 89976-A
Unable to display preview. Download preview PDF.
- 4.Jong, Y.-J., Thampy, K. G., and Barnes, Jr E. M. 1986. Ontogeny of GABAergic neurons in chick brain: studiesin vivo andin vitro. Develop. Brain Res. 25:83–90.Google Scholar
- 5.Langui, D., Sarhan, S., Devilliers, G., Pettmann, B., Delaunoy, J. P., Seiler, N., and Sensenbrenner, M. 1981. Synapse formation and development of neurotransmitter functions in neuronal cells from chick brain cultured in a serum-free,defined medium. Int. J. Dev. Neurosci. 6:137–147.Google Scholar
- 7.Mangoura, D., and Vernadakis, A. 1988. GABAergic neurons in cultures derived from three-, six-or eight-day-old chick embryo: a biochemical and immunocytochemical study. Develop. Brain Res. 40:25–35.Google Scholar
- 8.Shousboe, A., and Hertz, L. 1987. Primary cultures of GABAergic and glutamateric neurons as model systems to study neurotransmitter functions II. Developmental aspects. Pages 33–41, in Vernadakis, A (ed.), Model systems of development and aging of the nervous system, Martinus Nijhoff Publishing.Google Scholar
- 13.Beale, R., and Osborne, N. N. 1983. Selective uptake of tritiated glycine, GABA and D-aspartate by retinal cells in culture- a study using autoradiography and simultaneous immunofluorescence. Develop. Brain Res. 7:107–120.Google Scholar
- 15.Hamburger, V., and Hamilton, H. L. 1951. A series of normal stages in the development of the chick embryo. J. Morphol. 88:49–92.Google Scholar
- 18.Borg, J., Spitz, B., Hamel, G., and Mark, J. 1985. Selective culture of neurons from rat cerebral cortex: Morphological characterization, glutamate uptake and related enzymes during maturation in various culture media. Develop. Brain Res. 18:37–49.Google Scholar