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Neurochemical Research

, Volume 17, Issue 4, pp 333–337 | Cite as

On the activity of γ-aminobutyric acid and glutamate transporters in chick embryonic neurons and rat synaptosomes

  • Lena Lewin
  • Mats-Olof Mattsson
  • David K. Rassin
  • Åke Sellström
Original Articles

Abstract

The uptake of radioactive γ-aminobutyric acid (GABA) andd-aspartate and the effect of SKF 89976-A, a non-substrate inhibitor of the GABA transporter, on this uptake have been investigated. Neuronal cultures from eight-day-old chick embryos grown for three or six days in vitro, were used as a model. For comparison, we also used the P2-fraction from rat. Neuronal cultures grown for three and six days expressed high-affinity uptake systems for [3H]GABA and ford-[3H]aspartate with an increasing Vmax during this period. The lipophilic non-substrate GABA uptake inhibitor, SKF 89976-A, inhibited transporter mediated uptake of GABA both in cell cultures from chicken, and in P2-fractions from rat. The results also showed that SKF 89976-A was a poor inhibitor of the uptake ofd-aspartate. We found no non-saturable uptake ofd-aspartate.

Key Words

GABA d-aspartate glutamate primary neuronal culture rat synaptosomes SKF 89976-A 

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Copyright information

© Plenum Publishing Corporation 1992

Authors and Affiliations

  • Lena Lewin
    • 1
  • Mats-Olof Mattsson
    • 1
  • David K. Rassin
    • 2
  • Åke Sellström
    • 1
    • 3
  1. 1.Department of ZoophysiologyUniversity of UmeåUmeåSweden
  2. 2.Metabolic Laboratories, Department of PediatricsUniversity of Texas, Medical BranchGalvestonTexas
  3. 3.Swedish Defence Research EstablishmentUmeåSweden

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