Neurochemical Research

, Volume 15, Issue 6, pp 609–611 | Cite as

Regional distribution of DNA and RNA in rat brain: A sensitive determination using high-performance liquid chromatography with electrochemical detection

  • Tatsuo Nakahara
  • Makoto Hirano
  • Takashi Matsumoto
  • Toshihide Kuroki
  • Yoshinori Tatebayashi
  • Tetsuyuki Tsutsumi
  • Kouji Nishiyama
  • Hiroaki Ooboshi
  • Kaoru Nakamura
  • Hiroshi Yao
  • Akio Shiraishi
  • Michinori Waki
  • Hideyuki Uchimura
Original Articles

Abstract

DNA and RNA contents in 20 brain regions or nuclei of the rat were determined by a highly sensitive method using high-performance liquid chromatography with electrochemical detection. The high DNA and RNA contents were found in the hypothalamic nuclei, especially the median eminence-arcuate nucleus. These results may be available for the preparation of nucleic acids as the regional control.

Key Words

DNA RNA central nervous system electrochemical detection HPLC 

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Copyright information

© Plenum Publishing Corporation 1990

Authors and Affiliations

  • Tatsuo Nakahara
    • 1
  • Makoto Hirano
    • 2
  • Takashi Matsumoto
    • 2
  • Toshihide Kuroki
    • 2
  • Yoshinori Tatebayashi
    • 2
  • Tetsuyuki Tsutsumi
    • 2
  • Kouji Nishiyama
    • 2
  • Hiroaki Ooboshi
    • 2
  • Kaoru Nakamura
    • 2
  • Hiroshi Yao
    • 2
  • Akio Shiraishi
    • 3
  • Michinori Waki
    • 1
  • Hideyuki Uchimura
    • 2
  1. 1.Department of Chemistry, Faculty of ScienceKyushu UniversityFukuokaJapan
  2. 2.Center for Emotional and Behavioral DisordersHizen National Mental HospitalSagaJapan
  3. 3.Department of Biology, Faculty of ScienceKyushu UniversityFukuokaJapan

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