Induction of cerebroside synthesis in oligodendroglia
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Oligodendroglia function to produce myelin membranes which surround axons, enhancing saltatory conduction. Myelin consists of a multitude of condensed membranes which are rich in lipids with the major glycolipids, cerebrosides, being 25% of the total lipid. Thus a fully differentiated oligodendroglial cell that is producing myelin membranes would be actively synthesizing cerebrosides. Our laboratory has prepared and analyzed oligodendroglia from mature bovine brain, from neonatal rat brain, and from actively myelinating rat brain. Our studies suggest that the rat oligodendroglia in our culture systems are less differentiated than bovine cells in that they produce lower levels of cerebrosides. Addition of glucocorticoids, thyroid hormone, or retinoic acid all increased synthesis of cerebrosides in rat oligodendroglia. Ketone bodies were also somewhat stimulatory. Having no effect or causing dedifferentiation of the cells were 5-azacytidine and phorbol esters. Thus induction of cerebroside synthesis in oligodendroglia is complex and may involve many factors.
Key WordsOligodendroglia cerebrosides differentiation growth factors
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- 1.Poduslo, S. E., Miller, K., and McKhann, G. M., 1978. Metabolic properties of maintained oligodendroglia purified from brain. J. Biol. Chem. 253:1592–1597.Google Scholar
- 2.Poduslo, S. E., Curbeam, R., Miller, K., and Reier, P. 1985. Purification and characterization of cultures of oligodendroglia from rat brain. J. Neurosci. Res. 14:433–447.Google Scholar
- 3.Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J., 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265–275.Google Scholar
- 4.Poduslo, S. E., Miller, K., and Wolinsky, J., 1982. The production of a unique membrane by purified oligodendroglia in culture. Exper. Cell Res. 137:203–215.Google Scholar
- 5.Poduslo, S. E., and Miller, K., 1985. Levels of sulfatide synthesis distinguish oligodendroglia in different stages of maturation. Neurochem. Res. 10:1285–1297.Google Scholar
- 6.Evans, R. M. 1988. The steroid and thyroid hormone receptor superfamily. Science 240:889–895.Google Scholar
- 7.Robinson, A. M. and Williamson, D. H., 1980. Physiological roles of ketone bodies as substrates and signals in mammalian tissue. Phys. Rev. 60:143–187.Google Scholar
- 8.Kruh, J., 1982. Effects of sodium butyrate, a new pharmacological agent, on cells in culture. Mol. Cell Biochem. 42:65–82.Google Scholar
- 9.Lin, A. Y. C. and Chen, K. Y., 1985. Differential effects of the tumor promoter phorbol-12-myristate-13-acetate on the morphological and biochemical differentiation of N-18 mouse neuroblastoma cells. J. Cell Physiol. 125:387–392.Google Scholar
- 10.Rosoff, P. M., and Cantley, L. C. 1985. Lipopolysaccharide and phorbol esters induce differentiation but have opposite effects on phosphatidylinositol turnover and Ca2+ mobilization in 702/3 pre-B lymphocytes. J. Biol. Chem. 260:9208–9215.Google Scholar
- 11.Chapman, A. B., Knight, D. M., Dieckmann, B. S., and Ringold, G. M., 1984. Analysis of gene expression during differentiation of adipogenic cells in culture and hormonal control of the developmental program. J. Biol. Chem. 259:15548–15555.Google Scholar