Neurochemical Research

, Volume 20, Issue 4, pp 395–400 | Cite as

Dopaminergic parameters in the striatum and substantia nigra of seven strains of mice: Higher density in striatum of CAST compared to BALB mice

  • J. A. Richter
  • M. G. Brenneman
  • S. R. Dlouhy
  • B. Ghetti
Original Articles

Abstract

Tyrosine hydroxylase activity was assayed in microdissected substantia nigra and striata from seven strains of mice (BALB, CBA, YBR, WB, IS, MOLG, and CAST). In the substantia nigra where tyrosine hydroxylase activity is thought to be proportional to dopaminergic neuron number, only CBA had a different (lower) enzyme activity compared with BALB. However in the striatum, tyrosine hydroxylase activity was larger for IS, MOLG and CAST compared with BALB. Further investigation of the CAST striatum showed that dopamine content and dopamine uptake activity were also higher in comparison with BALB. All three dopaminergic parameters were larger because of lower protein levels in the CAST striatum. A lower absolute amount of glutamic acid decarboxylase activity in CAST versus BALB striatum was consistent with the possibility of a smaller CAST striatum. In contrast to dopamine, the serotonin content in CAST striatum was reduced in proportion to the decrease in protein content. We suggest that the CAST striatum is smaller than BALB striatum and is innervated by proportionally fewer serotoninergic terminals, but the amount of dopaminergic innervation of the CAST striatum is not altered by the size of the target.

Key Words

Mouse strains tyrosine hydroxylase nigrostriatal pathway dopamine serotonin glutamic acid hydroxylase 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Ross, R. A., Judd, A. B., Pickel, V. M., Joh, T. H., and Reis, D. J. 1976. Strain-dependent variations in number of midbrain dopaminergic neurones. Nature 264:654–656.Google Scholar
  2. 2.
    Reis, D. J., Baker, H., Fink, J. S., and Joh T. H. 1981. A genetic control of the number of dopamine neurons in mouse brain: Its relationship to brain morphology, chemistry, and behavior. Pages 215–229 in Gershon E. S., Matthysse, S., Breakefield, X. O., and Ciaranello, R. D. (eds.) Genetic Research Strategies for Psychobiology and Psychiatry. The Boxwood Press.Google Scholar
  3. 3.
    Triarhou, L. C., Norton, J., and Ghetti, B. 1988. Mesencephalic dopamine cell deficit involves areas A8, A9 and A10 in weaver mutant mice. Exp. Brain Res. 70:256–265.Google Scholar
  4. 4.
    Bostwick, J. R., and Le W.-D. 1991. A tyrosine hydroxylase assay in microwells using coupled nonenzymatic decarboxylation of dopa. Anal. Biochem. 192:125–130.Google Scholar
  5. 5.
    Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. 1951. Protein measurement with the folin phenol reagent. J. biol. Chem. 193:265–275.Google Scholar
  6. 6.
    Ingram, D. K. and Corfman, T. P. 1980. An overview of neurobiological comparisons in mouse strains. Neurosci. & Biobehav. Rev. 4:421–435.Google Scholar
  7. 7.
    Vadasz, C., Laszlovszky, I., DeSimone P. A., and Fleischer, A. 1992. Short Review. Genetic aspects of dopamine receptor binding in the mouse and rat brain: An overview. J. Neurochem. 59:793–808.Google Scholar
  8. 8.
    Dietrich, W., Katz, H., Lincoln, S. E., Shin, H.-S., Friedman, J., Dracopoli, N. C., and Lander, E. S. 1992. A genetic map of the mouse suitable for typing intraspecific crosses. Genetics 131:423–447.Google Scholar
  9. 9.
    Baker, H., Joh, T. H., and Reis, D. J. 1980. Genetic control of number of midbrain dopaminergic neurons in inbred strains of mice: Relationship to size and neuronal density of the striatum. Proc. Natl. Acad. Sci. USA 77:4369–4373.Google Scholar
  10. 10.
    Sved, A. F., Baker, H. A., and Reis, D. J. 1984. Dopamine systhesis in inbred mouse strains which differ in numbers of dopamine neurons. Brain Res. 303:261–266.Google Scholar
  11. 11.
    Boehme, R. E., and Ciaranello, R. D. 1981. Dopamine receptor binding in inbred mice: Strain differences in mesolimbic and nigrostriatal dopamine binding sites. Prod. Natl. Acad. Sci. USA 78:3255–3259.Google Scholar
  12. 12.
    Kanes, S. J., Hitzemann, B. A., and Hitzemann, R. J. 1993. On the relationship between D2 receptor density and neuroleptic-induced catalepsy among eight inbred strains of mice. J. Pharmacol. Exp. Therap. 267:538–547.Google Scholar
  13. 13.
    Shannon, H. E., Bemis, K. G., and Peters, S. C. 1991. Potency and efficacy of dopamine agonists in mouse strains differing in dopamine cell and receptor number. Pharmacol. Biochem. Behav. 40:103–107.Google Scholar
  14. 14.
    Suaudeau, C., Bousselmame, R., and Constentin, J. 1992. A different balance in the sensitivity of D1 and D2 dopamine receptors accounts for differences in apomorphine-induced hypothermic effects in Swiss and C3H mice. Neuropharmacology 31:1115–1119.Google Scholar
  15. 15.
    Sanghera, M. K., Fuchs, I., Weidmer-Mikhail, E., and Speciale, S. G. 1987. Met-enkephalin levels in midbrain dopamine regions of inbred mouse strains which differ in the number of dopamine neurons. Brain Res. 412:200–203.Google Scholar
  16. 16.
    Gerfen, C. R. 1992. The neostriatal mosaic: Multiple levels of compartmental organization in the basal ganglia. Annu. Rev. Neurosci. 15:285–320.Google Scholar
  17. 17.
    Tunnicliff, G., Wimer, C. C., and Wimer, R. E. 1973. Relationships between neurotransmitter metabolism and behaviour in seven inbred strains of mice. Brain Res. 61:428–434.Google Scholar

Copyright information

© Plenum Publishing Corporation 1995

Authors and Affiliations

  • J. A. Richter
    • 1
    • 2
    • 5
    • 6
  • M. G. Brenneman
    • 3
  • S. R. Dlouhy
    • 4
  • B. Ghetti
    • 2
    • 3
    • 4
    • 5
  1. 1.Department of Pharmacology and ToxicologyIndiana University School of MedicineIndianapolis
  2. 2.Department of PsychiatryIndiana University School of MedicineIndianapolis
  3. 3.Department of Pathology and Laboratory Medicine (Division of Neuropathology)Indiana University School of MedicineIndianapolis
  4. 4.Department of Medical and Molecular GeneticsIndiana University School of MedicineIndianapolis
  5. 5.Program in Medical NeurobiologyIndiana University School of MedicineIndianapolis
  6. 6.Institute of Psychiatric ResearchIndiana University School of MedicineIndianapolis

Personalised recommendations