Neurochemical Research

, Volume 20, Issue 4, pp 395–400 | Cite as

Dopaminergic parameters in the striatum and substantia nigra of seven strains of mice: Higher density in striatum of CAST compared to BALB mice

  • J. A. Richter
  • M. G. Brenneman
  • S. R. Dlouhy
  • B. Ghetti
Original Articles


Tyrosine hydroxylase activity was assayed in microdissected substantia nigra and striata from seven strains of mice (BALB, CBA, YBR, WB, IS, MOLG, and CAST). In the substantia nigra where tyrosine hydroxylase activity is thought to be proportional to dopaminergic neuron number, only CBA had a different (lower) enzyme activity compared with BALB. However in the striatum, tyrosine hydroxylase activity was larger for IS, MOLG and CAST compared with BALB. Further investigation of the CAST striatum showed that dopamine content and dopamine uptake activity were also higher in comparison with BALB. All three dopaminergic parameters were larger because of lower protein levels in the CAST striatum. A lower absolute amount of glutamic acid decarboxylase activity in CAST versus BALB striatum was consistent with the possibility of a smaller CAST striatum. In contrast to dopamine, the serotonin content in CAST striatum was reduced in proportion to the decrease in protein content. We suggest that the CAST striatum is smaller than BALB striatum and is innervated by proportionally fewer serotoninergic terminals, but the amount of dopaminergic innervation of the CAST striatum is not altered by the size of the target.

Key Words

Mouse strains tyrosine hydroxylase nigrostriatal pathway dopamine serotonin glutamic acid hydroxylase 


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Copyright information

© Plenum Publishing Corporation 1995

Authors and Affiliations

  • J. A. Richter
    • 1
    • 2
    • 5
    • 6
  • M. G. Brenneman
    • 3
  • S. R. Dlouhy
    • 4
  • B. Ghetti
    • 2
    • 3
    • 4
    • 5
  1. 1.Department of Pharmacology and ToxicologyIndiana University School of MedicineIndianapolis
  2. 2.Department of PsychiatryIndiana University School of MedicineIndianapolis
  3. 3.Department of Pathology and Laboratory Medicine (Division of Neuropathology)Indiana University School of MedicineIndianapolis
  4. 4.Department of Medical and Molecular GeneticsIndiana University School of MedicineIndianapolis
  5. 5.Program in Medical NeurobiologyIndiana University School of MedicineIndianapolis
  6. 6.Institute of Psychiatric ResearchIndiana University School of MedicineIndianapolis

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