Neurochemical Research

, Volume 20, Issue 4, pp 391–394 | Cite as

Lack of effect of transient ischemia on ubiquitin conjugation

  • Takeshi Kamikubo
  • Takashi Hayashi
  • Kiyoshi Ohkawa
Original Articles


Ubiquitin-conjugating activities in the soluble fractions of gerbil cortex and hippocampus following transient ischemia were examined in vitro. Ten minutes of ischemia did not affect the ubiquitination of heat-denatured lysozyme both in the cortex and in the hippocampus. No reduction of the conjugating activities following ischemia was also confirmed using the partially purified ubiquitin conjugating enzymes from the cortex. These results indicate that protein ubiquitination might not be impaired following transient ischemia.

Key Words

Ubiquitin ischemia cortex hippocampus gerbil 


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Copyright information

© Plenum Publishing Corporation 1995

Authors and Affiliations

  • Takeshi Kamikubo
    • 1
    • 2
  • Takashi Hayashi
    • 2
  • Kiyoshi Ohkawa
    • 2
  1. 1.Department of NeurosurgeryJikei University School of MedicineTokyo 105Japan
  2. 2.Department of BiochemistryJikei University School of MedicineTokyo 105Japan

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