A phosphoinositide-linked peptide response in astrocytes: Evidence for regional heterogeneity
A phosphoinositide-linked peptide response in cultured rat astrocytes was studied by measuring the accumulation of [3H]inositol phosphates in the presence of lithium. Cultures derived from cortex, cerebellum and spinal cord each showed a unique pattern or degree of stimulation to a panel of neuropeptides. Cortical and cerebellar astrocytes were similar, responding to bradykinin, oxytocin, vasopressin, eledoisin and neurokinin β, whereas spinal cord astrocytes were stimulated by substance P, bradykinin, eledoisin, and neurokinins α and β. These observations are evidence in favour of regional specialisations of astrocytes which may respond uniquely to peptidesreleased by particular populations of neurons.
Key WordsAstrocytes neuropeptides phosphoinositide metabolism
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- 5.Chneiweiss, H., Glowinski, J., and Prémont, J. 1985a. Vasoactive intestinal polypeptide receptors linked to an adenylate cyclase and their relationship with biogenic amide- and somatostatin-sensitive adenylate cyclases on central neuronal and glial cells in primary cultures. J. Neurochem. 44:779–786.PubMedGoogle Scholar
- 9.Goldstein, R. H., and Polgar, J. 1982. The effect and interaction of bradykinin and prostaglandin on protein and collagen production by lung fibroblasts. J. Biol. Chem. 259:8630–8633.Google Scholar
- 13.Henn, F. A. and Henn, S. W. 1980. The psychopharmacology of astroglial cells. Prog. in Neurobiol. 15:1–17.Google Scholar
- 14.Hertz, L., and Richardson, R. S. 1984. Is neuropharmacology merely the pharmacology of neurons—or are astrocytes important too? TIPS, 5:272–276.Google Scholar
- 16.Jan, Y., and Jan, L. 1983. An LHRH-like peptidergic neurotransmitter capable of action at a distance in autonomic ganglia. TINS 6:320–324.Google Scholar
- 19.Keller, M., Jackirsch, R., Seregi, A., and Hertting, G. 1985. Comparison of prostanoid forming capacity on neuronal and astroglial cells in primary cultures. Neurochem. Int. 7:655–665.Google Scholar
- 20.Kenny, J. 1986. Cell surface peptidases are neither peptidenor organ-specific. TIBS, 11:40–42.Google Scholar
- 23.Kuhar, M. J. 1985. The mis match problem in receptor matching studies. TINS, 8:190–191.Google Scholar
- 26.Nilaver, G., Zimmerman, E. A., Wilkins, J., Hoffman, D., and Silverman, A-J. 1980. Magnocellular hypothalamic projections to the lower brain stem and spinal cord of the rat. Neuroendocrinol. 30:150–158.Google Scholar
- 35.Van Calker, D., and Hamprecht, B. 1980. Effects of neurohormones on glial cells. Pages 31–67,in Federoff, S. and Hertz, L. (eds.) Advances in Cellular Neurobiology Vol. 1. Academic Press, New York.Google Scholar
- 38.Van Leeuwen, F. W., Pool, C. W., and Sluiter, A. A. 1983. Enkephalin immunoreactivity in synaptoid elements on glial cells in the rat neural lobe. Neurosci. 8:229–241.Google Scholar