Neurochemical Research

, Volume 19, Issue 11, pp 1363–1368 | Cite as

Insulin-like growth factor binding protein-1 is pre-synaptic at mouse neuromuscular synapses and is transported in nerve

  • Jianxin Ma
  • Shi X. Yang
  • Gilbert J. Ho
  • Barry W. Festoff
Original Articles

Abstract

In a previous study, we localized insulin-like growth factor binding protein 1 (IGFBP-1) to mouse neuromuscular junctions, and intramuscular nerves. To determine if pre-synaptic accumulation of IGFBP-1 occurred, we used double ligation of sciatic nerve in adult mice at different time points. IGFBPs were deteced by Western ligand blot (WLB) with125I-IGF-I. WLB and Western immunoblot (WIB) analysis of extracts from double-ligated nerves showed a delayed (6 days) increase of IGFBP-1 in the soluble fraction between the ligatures and distal to the distal ligature. For comparison we evaluated transport of neurofilament components, using WIB and confirmed the primarily anterograde transport of these intraaxonal proteins. These data suggest that expression of IGFBP-1 is both by activated Schwann cells as well as retrograde axonal transport with likely entry into the axon at the synapse.

Key Words

Neurotrophic paracrine regeneration nerve muscle ligation transport diabetes neuropathy ALS 

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References

  1. 1.
    D'Ercole, A. J., Stiles, A. D., and Underwood, L. E. 1984. Tissue concentrations of somatomedin C: further evidence for multiple sites of synthesis and paracrine or autocrine mechanisms of action. Proc. Natl. Acad. Sci. USA 81:935–939.PubMedGoogle Scholar
  2. 2.
    Baskin, D. G., Wilcox, B. J., Figlewitz, D. P., Dorsa, D. M. 1988. Insulin and insulin-like growth factors in the CNS. Trends Neurosci. 11:608–612.Google Scholar
  3. 3.
    Baxter, R. C., and Martin, J. L. 1989. Binding proteins for insulin-like growth factors: structure, regulation, and function. Prog. Growth Factor Res. 1:49–68.PubMedGoogle Scholar
  4. 4.
    Blum, W. F., Jenne, E. W., Reppin, F., Kietzmann, K., Ranke, M. B. and Bierich, J. R. 1989. Insulin-like growth factor I (IGF-I)-binding protein complex is a better mitogen than free IGF-I. Endocrinol. 25:766–772.Google Scholar
  5. 5.
    McGuire, W. L., Jr., Jackson, J. G., Figueroa, J. A., Shimasaki, S., Powell, D. R. and Yee, D. 1992. Regulation of Insulin-like growth factor-binding protein (IGFBP) expression by breast cancer cells: use of IGFBP-1 as an inhibitor of insulin-like growth factor action. J. National Cancer Institute 84:1336–1341.Google Scholar
  6. 6.
    Andress, D. L., and Birrbaum, R. S. 1992. Human osteoblastderived insulin-like growth factor (IGF) binding protein-5 stimulates osteoblast mitogenesis and protentiates IGF action. J. Biol. Chem. 267(31):22467–72.PubMedGoogle Scholar
  7. 7.
    Shimasaki, S., and Ling, N. 1991. Identification and molecular characterization of insulin-like growth factor binding proteins (IGFBP-1,-2,-3,-4,-5, and-6). Prog. Growth Factor Res.3:243–266.PubMedGoogle Scholar
  8. 8.
    Bondy, C. A. 1991. Transient IGF-I gene expression during the maturation of functionally related central projection neurons. J. Neurosci. 11:3442–3455.PubMedGoogle Scholar
  9. 9.
    Tollefsen, S. E., Lajara, R., MeCusker, R. H., Clemmons, D. R. and Rotwein, P. 1989. Insulin-like growth factors (IGF) in muscle development. Expression of IGF-I, the IGF-I receptor, and an IGF binding protein during myoblast differentiation. J. Biol. Chem. 264:13810–13817.PubMedGoogle Scholar
  10. 10.
    Hantaï, D., Rao, S. J., and Festoff, B. W. 1990. Rapid neural regulation of muscle urokinase-like plasminogen activator as defined by nerve crush. Pro. Natl. Acad. Sci. 87:2926–2930.Google Scholar
  11. 11.
    Ma, J., Yang, S. X., Ho, G. J. and Festoff, B. W. 1994. Insulin-like growth factor binding protein-1 at mouse neuromuscular synapses. Synapse. 17:225–229.PubMedGoogle Scholar
  12. 12.
    Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (Lond.) 227:680–685.Google Scholar
  13. 13.
    Hossenlopp, P., Seurin, D., Segovia-Quinson, B., Hardouin, S., and Binoux, M. 1986. Analysis of serum insulin-like growth factor binding proteins using Western Blotting: Use of the method for titration of the binding proteins and competitive binding studies. Anal. Biochem. 154:138–143.PubMedGoogle Scholar
  14. 14.
    Festoff, B. W., Rao, J. S., and Hantaï, D. 1991. Plasminogen activators and inhibitors in the neuromuscular system: III. The serpin protease nexin I is synthesized by muscle and localized at neuromuscular synapse. J. Cell. Physiol. 147:76–86.PubMedGoogle Scholar
  15. 15.
    Domingo, A., and Marco, R. 1989. Visualization under ultraviolet light enhances 100-fold the sensitivity of peroxidase-stained blots. Anal. Biochem. 182:176–181.PubMedGoogle Scholar
  16. 16.
    Wu, J. S., Hogan, G. R., and Morris, J. D. 1985. Modified methods for preparation of cryostal sections of skeletal muscle. Muscle & Nerve, 8:664–666.Google Scholar
  17. 17.
    Póvoa, G., Enberg, G., Jörnvall, H., and Hall, K. 1984. Isolation and characterization of a somatomedin-binding protein from midterm human amniotic fluid. Eur. J. Biochem. 144:199–204.PubMedGoogle Scholar
  18. 18.
    Brinkman, A., Groffen, C. A., Kortleve, D. J., Geurts van Kessel, A., and Drop, S. L. 1988. Isolation and characterization by a cDNA encoding the low molecular weight insulin-like growth factor binding protein (IGFBP-1). EMBO J. 7:2417–2423.PubMedGoogle Scholar
  19. 19.
    Ocrant, I. 1993. Insulin-like growth factor binding proteins in nervous tissue-derived cells. Ann. NY. Acad. Sci. 692:44–50.PubMedGoogle Scholar
  20. 20.
    Ocrant, I., Fay, C. T., and Pormelee, J. T. 1990. Characterization of insulin-like growth factor binding protein produced in rat central nervous system. Endocrinol. 127:1260–1267.Google Scholar
  21. 21.
    Brar, A. K., and Chernausek, S. D. 1993. Localization of insulin-like growth factor binding protein-4 expression in the developing and adult rat brain: Analysis by in situ hybridization. J. Neurosci. Res. 35:103–114.PubMedGoogle Scholar
  22. 22.
    Caroni, P., and Grandes, P. 1990. Nerve sprouting in innervated adult skeletal muscle induced by exposure to elevated levels of insulin-like growth factor. J. Cell. Biol. 110:1307–1317.PubMedGoogle Scholar
  23. 23.
    Crosby, S. R., Tsigös, C., Auderton, C.D., Gordon, C., Young, R. J., and White, A. 1992. Elevated plasma insulin-like growth factor binding protein-1 levels in type 1 insulin-dependent) diabetic patients with peripheral neuropathy. Diabetologia, 35:868–872.PubMedGoogle Scholar
  24. 24.
    Caroni, P. 1993. Activity-sensitive signaling by the muscle-derived insulin-like growth factors in the developing and regenerating neuromuscular system. Ann. N.Y. Acad. Sci. 692:209–222.PubMedGoogle Scholar
  25. 25.
    Blasi, J., Chapman, E. R., Link, E., Binz, T., Yamasaki, S., DeCamilli, P., Südhof, T. C., Niemann, H., and Jahn, R. 1993. Botulinum neurotoxin A selectively cleaves the synaptic protein SNAP-25. Nature 365:160–163.PubMedGoogle Scholar
  26. 26.
    Oyler, G. A., Higgins, G. A., Hart, R. A., Battenberg, E., Billingsley, M., Bloom, F. E., and Wilson, M. C. 1989. The identification of a novel synaptosomal-associated protein SNAP-25, differentially expressed by neuronal subpopulations. J. Cell Biology 109:3039–52.Google Scholar

Copyright information

© Plenum Publishing Corporation 1994

Authors and Affiliations

  • Jianxin Ma
    • 1
    • 2
  • Shi X. Yang
    • 1
    • 2
  • Gilbert J. Ho
    • 1
    • 2
  • Barry W. Festoff
    • 1
    • 2
  1. 1.Neurobiology Research LaboratoryVA Medical CenterKansas City
  2. 2.Department of NeurologyUniversity of Kansas Medical CenterKansas City

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