Neurochemical Research

, Volume 19, Issue 11, pp 1363–1368 | Cite as

Insulin-like growth factor binding protein-1 is pre-synaptic at mouse neuromuscular synapses and is transported in nerve

  • Jianxin Ma
  • Shi X. Yang
  • Gilbert J. Ho
  • Barry W. Festoff
Original Articles


In a previous study, we localized insulin-like growth factor binding protein 1 (IGFBP-1) to mouse neuromuscular junctions, and intramuscular nerves. To determine if pre-synaptic accumulation of IGFBP-1 occurred, we used double ligation of sciatic nerve in adult mice at different time points. IGFBPs were deteced by Western ligand blot (WLB) with125I-IGF-I. WLB and Western immunoblot (WIB) analysis of extracts from double-ligated nerves showed a delayed (6 days) increase of IGFBP-1 in the soluble fraction between the ligatures and distal to the distal ligature. For comparison we evaluated transport of neurofilament components, using WIB and confirmed the primarily anterograde transport of these intraaxonal proteins. These data suggest that expression of IGFBP-1 is both by activated Schwann cells as well as retrograde axonal transport with likely entry into the axon at the synapse.

Key Words

Neurotrophic paracrine regeneration nerve muscle ligation transport diabetes neuropathy ALS 


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Copyright information

© Plenum Publishing Corporation 1994

Authors and Affiliations

  • Jianxin Ma
    • 1
    • 2
  • Shi X. Yang
    • 1
    • 2
  • Gilbert J. Ho
    • 1
    • 2
  • Barry W. Festoff
    • 1
    • 2
  1. 1.Neurobiology Research LaboratoryVA Medical CenterKansas City
  2. 2.Department of NeurologyUniversity of Kansas Medical CenterKansas City

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