Abstract
The distribution of porphyrin metabolites (uro-7-6-5-copro-3-proto-heme) from 10−3 M delta-aminolevulinic acid (ALA) in neuronal and glial primary cultures was examined by an isotopic technique. In both cell types, 8–9% of the total production (cell + medium) was found in the cells and more than 90% in the medium. In both the cells and the medium the predominant porphyrin fraction was uro + 7 carboxylic. The heme fraction of the cells was also very high. Through the exclusion of ALA-synthetase activity, the “Uroporphyria-like-model” of “hepatic porphyria” was demonstrated.
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Durkó, I., Juhász, A. In vitro distribution of porphyrin metabolites from 10−3 M delta-aminolevulinic acid in primary neural tissue cultures. Neurochem Res 12, 465–468 (1987). https://doi.org/10.1007/BF00972299
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DOI: https://doi.org/10.1007/BF00972299