Neurochemical Research

, Volume 13, Issue 1, pp 83–86 | Cite as

Decreased calmodulin kinase activity after status epilepticus

  • Jeff Bronstein
  • Debora Farber
  • Claude Wasterlain
Original Articles

Abstract

Status epilepticus was induced in paralyzed, ventilated rats using bicuculline and was maintained for 50 to 120 minutes. Cerebral cortex, hippocampus, and cerebellum were assayed for calmodulin kinase II activity in vitro using [γ-32P]ATP and polyacrylamide gel electrophoresis. Seizures resulted in a 3.2 fold decrease in calmodulin kinase activity in crude synaptic membranes of cortex and in a 8.2 fold decrease in hippocampal membranes. Cytosolic calmodulin kinase activity was slightly increased in rats in status epilepticus but statistical significance was not reached. Status epilepticus did not affect calcium/calmodulin-dependent kinase activity in cerebellar membranes or cytosol. These data suggest that intense firing associated with continuous seizure activity decreases calmodulin kinase activity in cortical and hippocampal synaptic membranes, which may result in altered neuronal excitability.

Key Words

Calcium calmodulin kinase protein phosphoylation epilepsy status epilepticus 

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Copyright information

© Plenum Publishing Corporation 1987

Authors and Affiliations

  • Jeff Bronstein
    • 1
  • Debora Farber
    • 2
  • Claude Wasterlain
    • 1
    • 3
  1. 1.Department of NeuroscienceUCLA School of MedicineLos Angeles
  2. 2.Department of OphthalmologyUCLA School of MedicineLos Angeles
  3. 3.Department of NeurologyUCLA School of MedicineLos Angeles

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