Neurochemical Research

, Volume 13, Issue 8, pp 693–697 | Cite as

Identification of calcium binding proteins from brain

  • Blake W. Moore
Article

Abstract

A procedure was worked out for purification and identification of calcium-binding proteins from bovine brain using Ca2+-dependent, reversible binding to a hydrophobic support, phenyl-Sepharose, as the method of isolation. These proteins could be visualized during and after their separation by running them on non-denaturing polyacrylamide gels, blotting to Zeta-probe paper, and autoradiographing with45Ca2+. About 24 polypeptides could be seen in this fraction on SDS (Laemmli) gels and about 8–10 native, Ca2+-binding proteins could be seen on non-denaturing gels and on blots of their 45Ca2+ autoradiographs. Some of these proteins could be purified further by chromatography on DEAE-Sephacel and still retain their45Ca2+-binding activity.

Key Words

Calcium binding protein isolation purification 

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Copyright information

© Plenum Publishing Corporation 1988

Authors and Affiliations

  • Blake W. Moore
    • 1
  1. 1.Department of PsychiatryWashington University School of MedicineSt. Louis

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