Neurochemical Research

, Volume 12, Issue 11, pp 977–983 | Cite as

Long-term persistence of GAD activity in injured crayfish CNS tissue

  • Robert M. Grossfeld
  • Douglas B. Hansen
Original Articles


Crayfish CNS fibers were isolated in vivo from their cell bodies, from cellular connections in the CNS, and from peripheral sensory and effector cells. The glutamic acid decarboxylase (GAD) activity of the experimental tissues was about half of that of the sham-operated and unoperated control tissues by two weeks after surgery and remained at about that level during the ensuing six weeks. During that time, there was no significant behavioral, electrophysiological, or histological evidence of regeneration of nerve fibers across the lesion sites. The crush-isolated connectives possessed many intact axon profiles and nonneuronal cell nuclei. The long-term persistence of GAD activity in the injured CNS tissue may reflect the involvement of glial cells in maintaining neurotransmitter levels.

Key words

CNS injury GAD glia crayfish 


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Copyright information

© Plenum Publishing Corporation 1987

Authors and Affiliations

  • Robert M. Grossfeld
    • 1
  • Douglas B. Hansen
    • 1
  1. 1.Zoology Dept.North Carolina State UniversityRaleigh

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