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Regional distribution of dopamine, 5-hydroxytryptamine, and noradrenaline in the rat vas deferens

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Abstract

The concentrations of dopamine (DA), 5-hydroxytryptamine (5-HT) and noradrenaline (NA) in the rat vas deferens divided in eight or four sections were determined by high performance liquid chromatography with electrochemical detection. Dopamine and NA had the same regional distribution; their concentrations were maximal near the prostatic end and decreased towards the epididymis. The concentration of 5-HT also decreased from the prostatic to the epididimal end, but 5-HT did not follow the same regional distribution as DA and NA. Reserpine (0.02 or 0.2 mg/kg, i.p., 24 hr) and 6-hydroxydopamine (2×80 mg/kg, i.v., 6 days) decreased the contents of DA and NA; the concentrations of both amines were modified to a similar extent. Reserpine also diminished the content of 5-HT. Pargyline (200 mg/kg, i.p., 2 hr) increased the concentration of 5-HT whilep-chlorophenylalanine (300 mg/kg, oral, 3 days) decreased the contents of the amine in some sections of the vas deferens. This study suggests that DA and NA co-exist in the same sympathetic neurons. Some of the 5-HT could be stored in mast cells as previously proposed, but the finding that tissue content of 5-HT changes after inhibiting the deamination or synthesis of the amine suggests that other source(s) of 5-HT distinct from mast cells exist in the rat vas deferens.

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Author notes

  1. Stella Maris Celuch (Career Investigator)

    Present address: Consejo de Investigaciones Científicas y Técnicas, ININFA, Junín 956, 5°P, 1113, Buenos Aires, Argentina

Authors and Affiliations

  1. Neuropsychiatric Research Unit, University of Saskatchewan, Medical Research Building, S7N OWO, Saskatoon, Saskatchewan, Canada

    Stella Maris Celuch (Career Investigator) & B. Duff Sloley

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  1. Stella Maris Celuch
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  2. B. Duff Sloley
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Celuch, S.M., Sloley, B.D. Regional distribution of dopamine, 5-hydroxytryptamine, and noradrenaline in the rat vas deferens. Neurochem Res 13, 967–972 (1988). https://doi.org/10.1007/BF00970770

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