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A gas chromatographic method for the determination of inositol monophosphates in rat brain

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Abstract

Regional levels of cerebral inositol-1-phosphate (Ins1P), an intermediate in phosphoinositide (PI) cycle, were readily detected with a new gas chromatographic (GC) method. GC analysis of trimethylsilyated Ins1P and myo-inositol-2-phosphate with a fused silica capillary SE-30 column and flame ionization detection was linear at picomolar range (pmol/μl) with a sensitivity to a level of 2 pmol. Also, inositol monophosphates and glucose-6-phosphate are separated in unstimulated brain tissue. The mean recovery of the method is 98±5.2%. Ins 1P levels were higher in frontal than in caudal regions in control brains. Lithium treatment increased the levels of Ins1P throughout the brain but mostly in frontal brain regions and in the hippocampus. The present GC assay to measure the accumulation of Ins1P, an index for the activity of PI signalling, may be suitable for exploring regional differences in cerebral receptor-coupled PI signalling in vivo.

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Author notes

  1. Maija-Riitta Hirvonen

    Present address: Department of Environmental Hygiene and Toxicology, National Public Health Institute, P. O. Box 95, SF-70701, Kuopio, Finland

Authors and Affiliations

  1. Department of Industrial Hygiene, University of Kuopio, P.O. Box 6, SF-70211, Kuopio, Finland

    Maija-Riitta Hirvonen & Heikki Lihtamo

  2. Department of Environmental Hygiene and Toxicology, National Public Health Institute, P. O. Box 95, SF-70701, Kuopio, Finland

    Kai Savolainen

Authors
  1. Maija-Riitta Hirvonen
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  2. Heikki Lihtamo
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  3. Kai Savolainen
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Hirvonen, MR., Lihtamo, H. & Savolainen, K. A gas chromatographic method for the determination of inositol monophosphates in rat brain. Neurochem Res 13, 957–962 (1988). https://doi.org/10.1007/BF00970768

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