To investigate the incorporation of essential fatty acids into myelin components, 24-day-old rabbits were injected intracerebrally with [14C]linoleate, [14C]linolenate, or [3H]Myristate for comparison. Animals were killed 22 hr later and myelin was isolated. [3H]myristate labeled all myelin lipids including monogalactosyl diglyceride, with the exception of sulfatides. With14C-essential fatty acids, only glycerophospholipids were efficiently labeled and their specific activities were in the following decreasing orders: PC>PI>PE>PS with [14C]linoleate, and PE>PC>PI=PS with [14C]linolenate. Among myelin proteins, PLP and DM-20 were labeled with all 3 precursors. PLP was purified from myelin labeled with14C-essential fatty acids. The label was then cleaved from the protein by alkaline methanolysis and was identified as a dienoic ([14C]linoleate) or a tetraenoic ([14C]linolenate) fatty acid. MBP was not labeled with [3H]myristate, but was slightly labeled with both14C-essential fatty acids. The signification of the latter result is discussed.
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high-performance thin-layer chromatography
myelin basic protein
phosphatidylethanolamine and ethanolamine plasmalogens
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Bürgisser, P., Matthieu, JM. In vivo labeling of myelin lipids and proteolipid protein with [3H]myristate, [14C]linoleate, and [14C]linolenate. Neurochem Res 14, 91–96 (1989). https://doi.org/10.1007/BF00969764
- Myelin lipids
- proteolipid protein
- myristic acid
- linoleic acid
- lmolenic acid
- myelin basic protein