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Uptake and K+-stimulated release of [14C]glycine from frog retinal synaptosomal fractions

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Abstract

The uptake of [14C]glycine and the effect of depolarizing potassium concentrations on its release was investigated in the whole frog retina and its synaptosomal fractions. The uptake of [14C]glycine in retina and synaptosomal fractions was found to be saturable as well as energy and Na+-dependent. TheK m value for glycine uptake was found to be 46 μM for P2 fraction and 100 μM for P1 fraction, with aV max of 3.5 and 3.8 nmol/mg protein/min respectively. The release of [14C]glycine from P1 and P2 synaptosomal fractions was markedly increased by raising potassium concentration in the medium, in a partially Ca2+-dependent manner. Evoked glycine release was 50% reduced when calcium was omitted from the medium. The K+-stimulated release of glycine from P2 fraction was significantly reduced in the presence of TTX. The cellular origin of the P1 and P2 synaptosomal fractions releasing glycine is discussed.

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  1. Dept. de Neurociencias, Instituto de Fisiologia Celular, Universidad Nacional Autónoma de México, Apartado Postal 70-600, 04510, Mexico, D. F., México

    Rocío Salceda

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  1. Rocío Salceda
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Salceda, R. Uptake and K+-stimulated release of [14C]glycine from frog retinal synaptosomal fractions. Neurochem Res 14, 49–54 (1989). https://doi.org/10.1007/BF00969757

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