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Light exposure stimulates arachidonic acid metabolism in intact rat retina and isolated rod outer segments

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Abstract

This paper presents evidence that short-term exposure to light increases synthesis of hydroxyei-cosatetraenoic acid (HETE) and prostaglandin D2 (PGD2) and stimulates the uptake and metabolism of 20∶4 in phospholipids and triacylglycerols in rat retina. There was a time-dependent increase in incorporation of 1-14C-20∶4 into glycerolipids in both dark-adapted and light-exposed groups. Exposure to light for 15 or 30 min enhanced the acylation of 20∶4 into phosphatidylcholine, phosphatidylinositol, phosphatidylethanolamine and triacylglycerols. In the light-exposed groups there was a large increase in the conversion of 20∶4 to leukotriene B4, two diHETEs, 5-HETE, 15-HETE, and PGD2. The stimulation of HETE synthesis by light could be due to early stages of light-induced lipid peroxidation in visual cells. To examine this, we studied peroxidation of 20∶4 in isolated rod outer segments (ROS). There was more oxidation of 20∶4 in light-exposed ROS, as compared to ROS incubated in the dark. Vitamin E and nordihydroguaiaretic acid inhibited the light-induced formation of some of these products. The data indicate that photo-oxidation of 20∶4 in ROS is accompanied by enzymatic oxygenation that is stimulated by light. Increased production of HETEs and PGD2 may be a consequence of the light-induced stimulation of the metabolism of 20∶4 in membrane phospholipids in the retina.

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Author notes

  1. Dale L. Birkle

    Present address: Department of Pharmacology, West Virginia University Health Sciences Center North, 26506, Morgantown, WV

Authors and Affiliations

  1. Louisiana State University Eye Center, 2020 Gravier Street, 70112, New Orleans, Louisiana

    Dale L. Birkle & Nicolas G. Bazan

  2. Eye, Ear, Nose and Throat Hospital, 70112, New Orleans, Louisiana

    Dale L. Birkle & Nicolas G. Bazan

Authors
  1. Dale L. Birkle
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  2. Nicolas G. Bazan
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Birkle, D.L., Bazan, N.G. Light exposure stimulates arachidonic acid metabolism in intact rat retina and isolated rod outer segments. Neurochem Res 14, 185–190 (1989). https://doi.org/10.1007/BF00969637

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