Abstract
Rat brains allowed to autolyze in situ for 12 h selectively lost myelin proteins. Basic proteins are markedly decreased, but DM-20 (1) and proteolipid proteins also are lost from the myelin of developing and mature rat brain. At room temperature there is a 50% decrease in the concentration of basic protein in myelin isolated from 15-day-old rats. Reducing the ambient temperature to 0°C reduces the loss to 20%. Similar but less marked changes occur in the brains of adult animals. The molar ratios of cholesterol, phospholipids, and glycolipids are unaffected by autolysis, and there is also no change in the specific activity of the myelin marker enzyme 2′,3′-cyclic nucleotide-3′-phosphohydrolase (E.C.3.1.4.37). Electron microscopic examination of the isolated myelin demonstrates multilammelar structure with intraperiod lines.
Similar content being viewed by others
References
Agrawal, H. C., Burton, R. M., Fishman, M. A., Mitchell, R. F., andPrensky, A. L. 1972. Partial characterization of a new myelin protein band.J. Neurochem. 19:2083–2089.
Sammeck, R., andBrady, R. O. 1972. Studies of the catabolism of myelin basic proteins of the ratein situ andin vitro.Brain Res. 42:441–453.
Swanson, P. D., Harvey, F. H., andStahl, W. L. 1973. Subcellular fractionation of postmortem brain.J. Neurochem. 20:465–475.
Gray, E. G., andWhittaker, V. P. 1962. The isolation of nerve endings from brain: an electron-microscopic study of cell fragments derived by homogenization and centrifugation.J. Anat. (Lond.) 96:79–87.
Ansari, K. A., Hendrickson, H., Sinha, A. A., andRand, A. 1975. Myelin basic protein in frozen and unfrozen bovine brain. A study of autolytic changesin situ.J. Neurochem. 25:193–195.
Matthieu, T. M., Koellreutter, B., andGautier, E. 1976. Protein composition of CNS myelin afterin situ autolysis.Experientia: in press.
Fishman, M. A., Trotter, J. L., Alexander, A., Fujimoto, K., andAgrawal, H. C. 1976. Postmortem changes in CNS myelin proteinsin vivo.Trans. Am. Soc. Neurochem. 7:69 (Abs.).
Agrawal, H. C., Trotter, J. L., Burton, R. M., andMitchell, R. F. 1974. Metabolic studies on myelin: Evidence for a precursor role of a myelin subfraction.Biochem. J. 140:99–109.
Fishman, M. A., Agrawal, H. C., Alexander, A., Golterman, J., Martenson, R. E., andMitchell, R. F. 1975. Biochemical maturation of human myelin.J. Neurochem. 24:689–694.
Lowry, O. H., Rosebraugh, N. J., Farr, A. L., andRandall, R. J. 1951. Protein measurement with the Folin phenol reagent.J. Biol. Chem. 193:265–275.
Fujimoto, K., Roots, B. I., Burton, R. M., andAgrawal, H. C. 1976. Morphological and biochemical characterization of light and heavy myelin isolated from developing rat brain.Biochem. Biophys. Acta 409:235–248.
Weber, K., andOsborn, M. 1969. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.J. Biol. Chem. 244:4406–4412.
Allison, J. H., Agrawal, H. C., andMoore, B. W. 1974. Effect of N,N,N′,N′-tetramethylethylenediamine on the migration of proteins in SDS polyacrylamide gels.Anal. Biochem. 58:592–601.
Waehneldt, T. V. 1975. Ontogenetic study of a myelin-derived fraction with 2′,3′-cyclic nucleotide 3′-phosphohydrolase activity higher than that of myelin.Biochem. J. 151:435–437.
Martenson, R. E., andDeibler, G. E. 1975. Partial characterization of basic proteins of chicken, turtle, and frog central nervous system myelin.J. Neurochem. 24:79–88.
Marks, N., Grynbaum, A., andLajtha, A. 1976. The breakdown of myelin-bound proteins by intra- and extracellular proteases.Neurochem. Res. 1:93–111.
Einstein, E. R., Dalal, K. B., andCsejtey, J. 1970. Biochemical maturation of the central nervous system. II. Protein and proteolytic enzyme changes.Brain Res. 18:35–49.
Frey, H. J., Reikkinen, P. J., Arstila, A. U., Pelliniemi, T., andRinne, U. K. 1972. Maturational changes of CNS myelin with special reference to neutral proteinase activities.Brain Res. 45:165–173.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Fishman, M.A., Trotter, J.L. & Agrawal, H.C. Selective loss of myelin proteins during autolysis. Neurochem Res 2, 247–257 (1977). https://doi.org/10.1007/BF00969355
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00969355