Tunicamycin inhibits prostaglandin F2α receptor-mediated phosphoinositide hydrolysis in cultured rat astrocytes
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Effect of tunicamycin, an inhibitor of N-linked glycosylation, on prostaglandin (PG) F2α-stimulated phosphoinositide (PI) hydrolysis was examined in cultured rat astrocytes. Pretreatment of cultured astrocytes with tunicamycin (25–250 ng/ml) inhibited subsequent PGF2α (1 μM)-stimulated PI hydrolysis in concentration- and time-dependent manners. The inhibition completely recovered after removal of tunicamycin and re-incubation for 12 h. Tunicamycin pretreatment (100 ng/ml for 12 h) significantly blocked [35S]methionine incorporation into cultured astrocytes, but cell viability was not affected under the condition. Inhibitors of processing of N-linked sugar chains such as bromoconduritol, 1-deoxymannojirimycin, and swainsonine had no effect on PI response to PGF2α. These observations suggest that PGF2α receptor is N-linked glycosylated.
Key WordsProstaglandin F2α receptors tunicamycin N-Linked glycosylation phosphoinositide hydrolysis astrocytes
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- 1.Coleman, R. A., Kennedy, I., Humphrey, P. P. A., Bunce, K., and Lumley, P. 1989. Prostanoids and their receptors. Pages 643–714,in Hansch, C., Sammes, P. G., Taylor, J. B., and Emmett, J. C. (eds.), Comprehensive Medical Chemistry Vol. 3, Pergamon Press, Oxford.Google Scholar
- 6.Kitanaka, J., Hashimoto, H., Sugimoto, Y., Negishi, M., Aino, H., Gotoh, M., Ichikawa, A., and Baba, A. 1994. Cloning and expression of a cDNA for rat prostaglandin F2α receptor. Prostaglandins, in press.Google Scholar
- 20.Murphy, S., and Pearce, B. 1988. Functional receptors for neurotransmitters on astroglial cells. Neuroscience 22:381–394.Google Scholar