Neurochemical Research

, Volume 19, Issue 12, pp 1545–1550 | Cite as

Tunicamycin inhibits prostaglandin F receptor-mediated phosphoinositide hydrolysis in cultured rat astrocytes

  • Jun-ichi Kitanaka
  • Takako Hamano
  • Masayuki Gotoh
  • Hitoshi Hashimoto
  • Akemichi Baba
Original Articles


Effect of tunicamycin, an inhibitor of N-linked glycosylation, on prostaglandin (PG) F-stimulated phosphoinositide (PI) hydrolysis was examined in cultured rat astrocytes. Pretreatment of cultured astrocytes with tunicamycin (25–250 ng/ml) inhibited subsequent PGF (1 μM)-stimulated PI hydrolysis in concentration- and time-dependent manners. The inhibition completely recovered after removal of tunicamycin and re-incubation for 12 h. Tunicamycin pretreatment (100 ng/ml for 12 h) significantly blocked [35S]methionine incorporation into cultured astrocytes, but cell viability was not affected under the condition. Inhibitors of processing of N-linked sugar chains such as bromoconduritol, 1-deoxymannojirimycin, and swainsonine had no effect on PI response to PGF. These observations suggest that PGF receptor is N-linked glycosylated.

Key Words

Prostaglandin F receptors tunicamycin N-Linked glycosylation phosphoinositide hydrolysis astrocytes 


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Copyright information

© Plenum Publishing Corporation 1994

Authors and Affiliations

  • Jun-ichi Kitanaka
    • 1
  • Takako Hamano
    • 1
  • Masayuki Gotoh
    • 1
  • Hitoshi Hashimoto
    • 1
  • Akemichi Baba
    • 1
  1. 1.Department of Pharmacology, Faculty of Pharmaceutical SciencesOsaka UniversitySuita, OsakaJapan

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