Skip to main content
SpringerLink
Log in

KB-2796, a calcium channel blocker, ameliorates ischemic spinal cord damage in rabbits

  • Original Articles
  • Published:
Neurochemical Research Aims and scope Submit manuscript

Abstract

The effect of the calcium channel blocker (KB-2796) on metabolic and functional recovery in rabbit spinal cord after 20, 30, and 40 min ischemia and 4 days of recovery was investigated. The drug was given intraperitoneally in three different doses, 10, 20, or 50 mg/kg pre-or post-ischemia of 20, 30, or 40 min duration. Both higher doses 20 and 30 mg/kg completely recovered energy state and significantly improved neurological functions in the spinal cord following 20 and 30 min ischemia. Partial protection was observed even after 40 min ischemia. The protective effect of KB-2796 exceeds the effect of calcium blockers previously used in experimental spinal cord ischemia.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Siesjo, B. K. 1992. Pathophysiology and treatment of focal cerebral ischemia. J. Neurosurg. 77:337–354.

    PubMed  Google Scholar 

  2. Vaagenes, P., Cantadore, R., Safar, P., Moossy, J., Rao, G., Diven, W., Alexander, H., and Stezoski, W. 1984. Amelioration of brain damage by lidoflazine after prolonged ventricular fibrillation cardiac arrest in dogs. Crit. Care Med. 12:846–855.

    PubMed  Google Scholar 

  3. Deshpande, J. K., and Wieloch, T. 1986. Flunarizine, a calcium entry blocker, ameliorates ischemic brain damage in the rat. Anesthesiology 64:215–224.

    PubMed  Google Scholar 

  4. Steen, P. A., Gisvold, S. E., Milde, J. H., Newberg, L. A., Schauthauser, B. W., Lanier, W. L., and Michenfelder, J. D. 1985. Nimodipine improves outcome when given after complete cerebral ischemia in primates. Anesthesiology 62:406–414.

    PubMed  Google Scholar 

  5. Chavko, M., Kalincakova, K., Kluchova, D., and Nemoto, E. 1991. Blood flow and electrolytes in spinal cord ischemia. Exp. Neurol. 112:299–303.

    PubMed  Google Scholar 

  6. Young, W., and Flamm, E. S. 1982. Effect of high-dose corticosteroid therapy on blood flow, evoked potentials, and extracellular calcium in experimental spinal injury. J. Neurosurg. 57:667–673.

    PubMed  Google Scholar 

  7. Faden, A. I., Jacobs, T. P., Smith, G. P., Green, B., and Zivin, J. A. 1983. Neuropeptides in spinal cord injury: comparative experimental models. Peptides 4:631–634.

    PubMed  Google Scholar 

  8. Faden, A. I., Jacobs, T. P., and Smith, M. T. 1984. Evaluation of the calcium channel antagonist nimodipine in experimental spinal cord ischemia. J. Neurosurg. 60:796–799.

    PubMed  Google Scholar 

  9. Kanazawa, T., Morita, T., Harada, K., and Ito, K. 1987. Selective effect of KB-2796, a new calcium antagonist, on cerebral circulation in anesthetized dogs. Jpn. J. Pharmacol. 43:98P.

    Google Scholar 

  10. Ito, K., Yoshidomi, M., Ozaki, A., Yamashita, A., Hayashi, A., and Sukamoto, T. 1987. Effect of KB-2796 on cerebral hypoxia/ischemia and memory deficit in animal models. 10th Int. Congr. Pharmacol. Pages 716, Sydney.

  11. Yamashita, A., Hayashi, A., Ozaki, A., Sukamoto, T., and Ito, K. 1987. Effect of KB-2796 upon local cerebral glucose utilization in the rat brain. Jpn. J. Pharmacol. 43:98.

    PubMed  Google Scholar 

  12. Danielisova, V., Marsala, M., Chavko, M., and Marsala, J. 1990. Postischemic hypoxia improve metabolic and functional recovery of the spinal cord. Neurology 40:1125–1129.

    PubMed  Google Scholar 

  13. Anderson, D. K., Means, E. D., Waters, T. R., and Green, E. S. 1993. Susceptibility of feline spinal cord energy metabolism to severe incomplete ischemia. Neurology 33:722–731.

    Google Scholar 

  14. Folbergrova, J., Ljunggren, B., Norberg, K., and Siesjo, B. K. 1972. Influence of complete ischemia on glycolytic metabolites, citric acid intermediates, and associated amino acids in the rat cerebral cortex. Brain Res. 80:265–279.

    Google Scholar 

  15. Atkinson, D. E. 1968. The energy charge of the adenylate pool as a regulatory parameter. Interaction with feedback modifiers. Br. J. Pharmacol. 7:4030–4032.

    Google Scholar 

  16. Zivin, J. A., DeGirolami, U., and Hurwitz, E. L. 1982. Spectrum of neurological deficit in experimental CNS ischemia. A quantitative study. Arch. Neurol. 39:408–412.

    PubMed  Google Scholar 

  17. Yoshidomi, M., Hayashi, T., Abe, K., and Kogure, K. 1989. Effects of a new calcium channel blocker, KB-2796, on protein synthesis of the CA1 pyramidal cell and delayed neuronal death following transient forebrain ischemia. J. Neurochem. 53:1589–1594.

    PubMed  Google Scholar 

  18. Harada, K., Nakasu, Y., Matsuda, H., and Handa, J. 1987. Effect of KB-2796 on the regional cerebral blood flow. Comparison with the effect of flunarizine. Jpn. Pharmacol. Ther. 15:1499–1506.

    Google Scholar 

  19. Kanazawa, T., Kidooka, M., Matsuda, M., and Handa, J. 1986. Effects of 1-[bis(4-flurophenyl)methyl]-4-(2,3,4-trimethoxybenzyl) piperazine dihydrochloride, a new synthesized Ca2+ blocker KB-2796, on free fatty acid liberation in ischemic brain in rats. Arch. Jpn. Chir. 55:755–761.

    Google Scholar 

  20. Hansen, A. J., and Zeuthen, T. 1981. Extracellular ion concentrations during spreading depressing and ischemia in the brain cortex. Acta Physiol. Scand. 113:437–455.

    PubMed  Google Scholar 

  21. Gelbfish, J. S., Phillips, T., Rose, D. M., Wait, R., and Cunningham, J. N. 1986. Acute spinal cord ischemia: prevention of paraplegia with verapamil. Circulation 74:I-5–I-10.

    Google Scholar 

  22. Danielisova, V., and Chavko, M. 1992. Amelioration of ischemic spinal cord damage by postischemic treatment with propentofylline. Brain Res. 590:321–324.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Neurobiology, Department of Neurochemistry, Slovak Academy of Sciences, Ul. Vystavby, 04001, Kosice, Kosice, Slovak Republic

    Viera Danielisova & Mikulas Chavko

Authors
  1. Viera Danielisova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mikulas Chavko
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Danielisova, V., Chavko, M. KB-2796, a calcium channel blocker, ameliorates ischemic spinal cord damage in rabbits. Neurochem Res 19, 1503–1507 (1994). https://doi.org/10.1007/BF00968997

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00968997

Key Words

Search

Navigation