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Das Verhalten trypsinierter Plexus-chorioideus-Zellen in Gewebekulturen

Eine elektronenmikroskopische Untersuchung
  • K. Meller
  • H. H. Wagner
  • W. Breipohl
Article

Zusammenfassung

Die Plexus chorioidei der Seitenventrikel 10 Tage alter Hühnerembryonen wurden trypsiniert, kultiviert und anschließend licht- und elektronenmikroskopisch untersucht. Unmittelbar nach der Trypsinierung sind die Plexuszellen bei der Kultivation in flüßigen Medium als Einzelzellen anzutreffen. Nach 12stündiger Kulturzeit sieht man kleine Rosetten, deren Größe bis zum Alter von 36 Std noch zunimmt. Die isolierten Zellen und die Zellen der Rosetten weisen eine rege Zilientätigkeit auf, durch die sie in lebhafter Bewegung gehalten werden.

Mit der Zusammenlagerung der isolierten Zellen zu Gewebsverbänden geht gleichzeitig eine feinstrukturelle Differenzierung einher. Es treten Desmosomen, ein basales Labyrinth, eine Basalmembran und oft ein Ergastoplasma auf.

Trypsinized cells of the plexus chorioideus in tissue cultures

Summary

Plexus cells of 10 days old chicken embryos were trypsinized. After various periods of culturing the material was studied both light- and electronmicroscopically. Directly after dissociation most of the plexus cells are found to be separated from each other in the liquid medium. However after 12 hours of culturing the individual cells reaggregate themselves in small rosettes which enlarge until the stages of 36 hours. Simultaneously the aggregational process of previously dissociated cells is combined with an ultrastructural differentiation and desmosomes, basal labyrinth, basement membrane and ergastoplasm are formed.

Both the isolated cells and the rosettes exhibit quick rotational movements due to their ciliar action.

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Copyright information

© Springer-Verlag 1969

Authors and Affiliations

  • K. Meller
    • 1
  • H. H. Wagner
    • 1
  • W. Breipohl
    • 1
  1. 1.Institut für Histologie und Neuroanatomie der Universität GöttingenDeutschland

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