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Uptake of H3-5-hydroxytryptophan by noradrenergic nerves in the mouse pancreas studied with electron microscopic autoradiography

  • Lars E. Ericson
Article

Summary

The uptake and distribution of radioactivity in vascular adrenergic nerves in the mouse pancreas following the injection of tritiated 5-hydroxytryptophan was studied by means of electron microscopic autoradiography. Autoradiographic silver grains were found selectively accumulated over axonal profiles. Quantitative analysis revealed a characteristic intraneuronal distribution of the silver grains, most of which probably represent 5-hydroxytryptamine formed by decarboxylation from the labeled precursor. Thus, the grain density over adrenergic nerve terminals, containing a mixed population of vesicles and granules, was about 5 times higher than the grain density recorded over non-terminal axonal parts and at least 20 times higher than the grain density found over surrounding adventitial tissue and smooth muscle cells. This was interpreted as an evidence that 5-hydroxytryptamine was taken up and stored in adrenergic terminals.

Key-Words

Noradrenergic nerves Terminals Uptake of 5-hydroxytryptamine Electron microscopic autoradiography 

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References

  1. Aghajanian, G. K., Bloom, F. E.: Localization of tritiated serotonin in rat brain by electron-microscopic autoradiography. J. Pharmacol. exp. Ther.156, 23–30 (1970).Google Scholar
  2. Alm, P., Cegrell, L., Ehinger, B., Falck, B.: Remarkable adrenergic nerves in the exocrine pancreas. Z. Zellforsch.83, 178–186 (1967).PubMedGoogle Scholar
  3. Bak, J., Kim, J. H., Hassler, R.: Electron microscopic autoradiography for demonstration of pineal serotonin in rat. Z. Zellforsch.105, 167–175 (1970).PubMedGoogle Scholar
  4. Eccleston, D., Thoa, N. B., Axelrod, J.: Inhibition by drugs of the accumulation in vitro of 5-hydroxytryptamine in guinea-pig vas deferens. Nature (Lond.)217, 846–847 (1968).Google Scholar
  5. Ekholm, R., Ericson, L. E.: Monoaminergic mechanisms in the exocrine pancreas of the mouse studied with electron microscopic autoradiography. J. Ultrastruct. Res. in press (1970).Google Scholar
  6. Ericson, L. E.: Subcellular localization of 5-hydroxytryptamine in the parafollicular cells of the mouse thyroid gland. J. Ultrastruct. Res.31, 162–177 (1970).PubMedGoogle Scholar
  7. - Ekholm, R.: Autoradiographic localization of monoamines in pancreatic islets of the albino mouse. J. Ultrastruct. Res. in press (1970).Google Scholar
  8. Gershon, M. D., Ross, L. L.: Radioisotope studies of the binding, exchange and distribution of 5-hydroxytryptamine synthesized from its radioactive precursor. J. Physiol. (Lond.)186, 451–476 (1966a).Google Scholar
  9. — —: Location of sites of 5-hydroxytryptamine storage and metabolism by radioautography. J. Physiol. (Lond.)186, 477–492 (1966b).Google Scholar
  10. Hökfelt, T.: In vitro studies on central and peripheral monoamine neurons at the ultrastructural level. Z. Zellforsch.91, 1–74 (1968).PubMedGoogle Scholar
  11. Jaim-Etcheverry, G., Zieher, L. M.: Cytochemistry of 5-hydroxytryptamine at the electron microscope level: II. Localization in the autonomic nerves of the rat pineal gland. Z. Zellforsch.86, 393–400 (1968).PubMedGoogle Scholar
  12. — —: Ultrastructural cytochemistry and pharmacology of 5-hydroxytryptamine in adrenergic nerve endings: I. Localization of exogenous 5-hydroxytryptamine in the autonomic nerves of the rat vas deferens. J. Pharmacol. exp. Ther.166, 264–271 (1969).PubMedGoogle Scholar
  13. Lever, J. D., Spriggs, T. L. B., Graham, J. D. P.: A formal-fluorescence fine-structural and autoradiographic study of the adrenergic innervation of the vascular tree in the intact and sympathectomised pancreas of the cat. J. Anat. (Lond.)103, 15–34 (1968).Google Scholar
  14. Lichtensteiger, W., Mutzner, U., Langemann, H.: Uptake of 5-hydroxytryptamine and 5-hydroxytryptophan by neurons of the central nervous system normally containing catecholamines. J. Neurochem.14, 489–497 (1967).PubMedGoogle Scholar
  15. Malmfors, T.: Studies on adrenergic nerves. Acta physiol. scand.64, Suppl. 248 (1965).Google Scholar
  16. Maunsbach, A. B.: Absorption of I125-labeled homologous albumin by rat kidney proximal tubule cells. A study of microperfused single proximal tubules by electron microscopic autoradiography and histochemistry. J. Ultrastruct. Res.15, 197–241 (1966).PubMedGoogle Scholar
  17. Owman, Ch.: Sympathetic nerves probably storing two types of monoamines in the rat pineal gland. J. Neuropharmacol.2, 105–112 (1964).Google Scholar
  18. Salpeter, M. M., Bachmann, L.: Autoradiography with the electron microscope. I. A procedure for improving resolution, sensitivity and contrast. J. Cell Biol.22, 469–476 (1964).PubMedGoogle Scholar
  19. Sitte, H.: Morphometrische Untersuchungen an Zellen. In: Quantitative methods in morphology. (E. R. Weibel and H. Elias, eds.) p. 167–198. Berlin-Heidelberg-New York: Springer 1967.Google Scholar
  20. Snipes, R. L., Thoenen, H., Tranzer, J. P.: Fine structural localization of exogenous 5-HT in vesicles of adrenergic terminals. Experientia (Basel)24, 1026–1027 (1968).Google Scholar
  21. Taxi, J., Droz, B.: Etude de l'incorporation de noradrenalin-3H (NA-3H) et de 5-hydroxytryptophane-3H (5.HTP-3H) dans les fibres nerveuses du canal déferent et de l'intestin. C. R. Acad. Sci. (Paris)263, 1237–1240 (1966).Google Scholar
  22. Thoa, N. B., Axelrod, J., Eccleston, D.: Uptake and release of C14-serotonin in the noradrenergic neurons of the guinea pig vas deverens. Pharmacologist9, 251 (1967).Google Scholar
  23. Watari, N.: Fine structure of nervous elements in the pancreas of some vertebrates. Z. Zellforsch.85, 291–314 (1968).PubMedGoogle Scholar
  24. Zelander, T., Ekholm, R., Edlund, Y.: The ultrastructural organization of the rat exocrine pancreas. III. Intralobular vessels and nerves. J. Ultrastruct. Res.7, 84–109 (1962).PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1971

Authors and Affiliations

  • Lars E. Ericson
    • 1
  1. 1.Department of AnatomyUniversity of GöteborgGöteborgSweden

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