Neurochemical Research

, Volume 17, Issue 7, pp 723–727 | Cite as

Increase in nucleoside diphosphatase in rat brain striatum lesioned with kainic acid

  • Shuzo Miyamoto
  • Yoshihiro Matsuda
  • Shin-ichiro Sano
  • Hiroshi Shiraki
  • Hachro Nakagawa
Original Articles


The activity of ammoniagenesis from guanine nucleotides was found to increase significantly in rat brain after infusion of kainic acid into the striatum. Among the enzymes involved in degrading guanine nucleotides, nucleoside diphosphatase was markedly increased in the lesioned striatum. The enzyme activity began to increase 2 days after the infusion, and reached the maximum on the 13th day, the level being 4 times as high as that of the intact contralateral region. The increased activity was due to Type L enzyme, judging from its substrate specificity. Puromycin and cycloheximide inhibited this increase, indicating that the increased activity resulted from an increase in the net synthesis of the enzyme. These findings suggest that Type L NDPase might play some important roles in gliosis after neuronal lesion.

Key Words

Ammoniagenesis brain nucleoside diphosphatase kainic acid gliosis 


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Copyright information

© Plenum Publishing Corporation 1992

Authors and Affiliations

  • Shuzo Miyamoto
    • 1
  • Yoshihiro Matsuda
    • 1
  • Shin-ichiro Sano
    • 1
  • Hiroshi Shiraki
    • 1
  • Hachro Nakagawa
    • 1
  1. 1.Institute for Protein ResearchOsaka UniversitySuita, OsakaJapan

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