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Neurochemical Research

, Volume 17, Issue 11, pp 1057–1062 | Cite as

Autoradiography of muscarinic cholinergic receptors in cortical and subcortical brain regions of C57BL/6 and DBA/2 mice

  • C. Schwab
  • G. Brückner
  • T. Rothe
  • C. Castellano
  • A. Oliverio
Original Articles

Abstract

Mice of the inbred strains C57BL/6 and DBA/2 show strain-dependent behavioural differences which have been correlated with variations in brain cholinergic systems. In the present study, the density of muscarinic cholinergic receptors in both strains of mice was determined by autoradiographic methods using [3H]quinuclidinyl benzilate (QNB) and [3H]pirenzepine as ligands. C57BL/6 mice showed a significantly lower [3H]QNB binding level in the frontal cortex by one third as compared to DBA/2 mice. In the striatum and the cholinergic pontomesencephalic nucleus laterodorsalis tegmenti the [3H]QNB binding was lower in C57BL/6 by 28% and 31%, respectively. The [3H]pirenzepine binding level was found to be significantly higher in C57BL/6 temporal cortex (by 22%). These results are discussed in relation to interstrain differences in cholinergic cell density and in the activity of cholinergic enzymes.

Key Words

[3H]QNB [3H]pirenzepine autoradiography C57BL/6 mice DBA/2 mice 

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Copyright information

© Plenum Publishing Corporation 1992

Authors and Affiliations

  • C. Schwab
    • 1
  • G. Brückner
    • 1
  • T. Rothe
    • 1
  • C. Castellano
    • 2
  • A. Oliverio
    • 2
    • 3
  1. 1.Paul Flechsig Institute for Brain Research, Department of NeurochemistryUniversity of LeipzigLeipzig(FRG)
  2. 2.Istituto di Psicobiologia e Psicofarmacologia del CNRUniversità “La Sapienza”Roma(Italy)
  3. 3.Dipartimento di Genetica e Biologia MolecolareUniversità “La Sapienza”Roma(Italy)

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