Neurochemical Research

, Volume 17, Issue 10, pp 1041–1048 | Cite as

A factor derived from chick embryo retina which inhibits DNA synthesis of retina itself

  • Giuseppe Calvaruso
  • Renza Vento
  • Gennaro Taibi
  • Michela Giuliano
  • Giovanni Tesoriere
Original Articles


Chick embryo retinas contain a peptide factor that inhibits DNA synthesis in explants of chick embryo retina. The inhibitory factor, obtained by acid/ethanol extraction from 15-day-old chick embryo retinas, was partially purified by affinity chromatography on heparin-sepharose CL-6B and gel filtration on Sephadex G-100. The inhibitor reduced DNA synthesis with maximal effects observed in retinal explants from 7 to 8-day-old chick embryos. The inhibitory effect became apparent after 10 h of incubation and reached the maximum levels after 16 h. DNA-inhibiting activity was heat and acid-stable and was destroyed by trypsin and alkaline treatments. The inhibitory effect was observed in retinal explants incubated in a medium free froml-glutamine, and the addition of this compound to the medium reduced the inhibitory effect in a concentration-dependent manner.

Key Words

Chick embryo retina development inhibitory factors retinal explants DNA synthesis 


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Copyright information

© Plenum Publishing Corporation 1992

Authors and Affiliations

  • Giuseppe Calvaruso
    • 1
  • Renza Vento
    • 1
  • Gennaro Taibi
    • 1
  • Michela Giuliano
    • 1
  • Giovanni Tesoriere
    • 1
  1. 1.Institute of Biological ChemistryUniversity of PalermoPoliclinicoItaly

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