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Muscarinic receptor-mediated increase in ζ-PKC expression in SK-N-SH human neuroblastoma cells

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Abstract

Anti-peptide antibodies specific for each protein kinase C (PKC) isozyme were used to screen SKN-SH human neuroblastoma cells. These cells were found to express only α- and ζ-PKC. Stimulation of these cells with phorbol esters caused α-but not ζ-PKC to translocate from cytosolic to membrane fractions. Stimulation of these cells with carbachol, which releases inositol trisphosphate and diacylglycerol, caused a transient translocation of α-PKC but not of ζ-PKC. Carbachol did, however, cause a gradual increase in immunoreactive ζ-PKC which reached maximal values 10–20 min after stimulation. These results implicate ζ-PKC in a receptor-mediated signalling event.

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  1. Departments of Radiology and of Pharmacology, The George Washington University Medical Center, Roos Hall, Room 662, 20037, Washington, D.C., 20037

    Jesse Baumgold & Kimberly D. Dyer

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  1. Jesse Baumgold
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  2. Kimberly D. Dyer
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Baumgold, J., Dyer, K.D. Muscarinic receptor-mediated increase in ζ-PKC expression in SK-N-SH human neuroblastoma cells. Neurochem Res 19, 135–138 (1994). https://doi.org/10.1007/BF00966807

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