Neurochemical Research

, Volume 19, Issue 2, pp 111–115 | Cite as

Pharmacological characterization of glutamate binding sites in cultured cerebellar granule cells and cortical astrocytes

  • I. Holopainen
  • Pirjo Saransaari
  • S. S. Oja
Original Articles

Abstract

Membranes prepared from cerebellar granule cells and cortical astrocytes exhibited specific, saturable binding ofl-[3H]glutamate. The apparent binding constant K d was 135 nM and 393 nM and the maximal binding capacity Bmax 42 and 34 μmol/kg in granule cells and astrocytes, respectively. In granule cells the binding was strongly inhibited by the glutamate receptor agonists kainate, quisqualate, N-methyl-d-aspartate (NMDA),l-homocysteate and ibotenate, and the antagonistdl-5-aminophosphonovalerate. In astrocytes, only quisqualate among these was effective.l-Aspartate,l-cysteate,l-cysteinesulphinate and γ-d-glutamylglycine were inhibitors in both cell types. The binding was totally displaced in both cell types byl-cysteinesulphinate with IC50 in the micromolar range. In astrocytes the binding was also totally displaced by quisqualate, but in granule cells only partially by NMDA, kainate and quisqualate in turn. It is concluded from the relative potencies of agonists and antagonists in [3H]glutamate binding that cerebellar granule cells express the NMDA, kainate and quisqualate types of the glutamate receptor, while only the quisqualate-sensitive binding seems to be present in cortical astrocytes.

Key Words

Cerebellar granule cells cortical astrocytes excitatory amino acid binding glutamate receptor subtypes 

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Copyright information

© Plenum Publishing Corporation 1994

Authors and Affiliations

  • I. Holopainen
    • 1
  • Pirjo Saransaari
    • 2
  • S. S. Oja
    • 2
  1. 1.Department of Biochemistry and PharmacyUniversity of Åbo AkademiÅboFinland
  2. 2.Department of Biomedical SciencesUniversity of TampereTampereFinland

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