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Stimulation of mono- and diacylglycerol lipase activities by gangliosides in chicken neuronal cultures

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Abstract

Chicken neurons in culture display measurable activities of mono-and diacylglycerol lipases. Treatment of chicken neuronal cultures with gangliosides (10−8M to 10−5M) resulted in a time and dose dependent increase in monoacylglycerol lipase activity. The diacylglycerol lipase showed significant increase in specific activity before that of monoacylglycerol lipase. The increase was observed only up to 24 hours and no differences between diacylglycerol lipase activity of control and ganglioside treated cells were observed after 48 hours. The data indicate that the treatment of neurons with exogenous gangliosides affect the diglyceride metabolism in stimulating not only the enzymes catalyzing their production but also those involved in their catabolism.

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Abbreviations

CDM:

chemical defined medium

DTNB:

5,5′ Dithiobis-(2-nitrobenzoic acid)

LPC:

lysophosphatidylcholine

MOPS:

3-[N-Morpholino] propanesulfonic acid

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Authors and Affiliations

  1. Centre de Neurochemie du CNRS, Antenne de Cronenbourg BP 20 CR, 23 rue du Loess, 67037, Strasbourg Cedex, France

    Louis Freysz, Lloyd A. Horrocks, Raphaël Massarelli & Henri Dreyfus

  2. Department of Medical Biochemistry, The Ohio State University, 1645 Neil Avenue, 43210, Columbus, OH, USA

    Aklaq A. Farooqui

Authors
  1. Louis Freysz
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  2. Aklaq A. Farooqui
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  3. Lloyd A. Horrocks
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  4. Raphaël Massarelli
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  5. Henri Dreyfus
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Freysz, L., Farooqui, A.A., Horrocks, L.A. et al. Stimulation of mono- and diacylglycerol lipase activities by gangliosides in chicken neuronal cultures. Neurochem Res 16, 1241–1244 (1991). https://doi.org/10.1007/BF00966702

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  • DOI: https://doi.org/10.1007/BF00966702

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