Neurochemical Research

, Volume 12, Issue 10, pp 923–928 | Cite as

Distribution and characteristics of nerve growth factor binding on cholinergic neurons of rat and monkey forebrain

  • R. J. Riopelle
  • P. M. Richardson
  • V. M. K. Verge
Original Articles

Abstract

In sections of rat forebrain, perikarya labeled radioautographically with125I-NGF resembled cholinesterase-positive neurons in their distribution within striatum and basal forebrain. Neurons with NGF receptors were also visualized in radioautographs prepared from the basal forebrain of a cerebrus monkey. Present techniques fail to detect axons projecting from basal forebrain to hippocampus or cortex which have been shown to take up NGF selectively in retrograde transport studies. In studies with membrane-enriched preparations from rat, high-affinity binding of125I-NGF (half maximal saturation in the 15–30 pM range) was detected in basal forebrain and striatum; lower levels of high-affinity binding were seen in hippocampus and neocortex. The binding and molecular properties of these receptors are similar to those described in other NGF-responsive tissues. These observations are further evidence supporting a biological role for NGF on some forebrain cholinergic neurons in adult rat.

Key Words

NGF receptors CNS cholinergic neurons 

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References

  1. 1.
    Schwab, M. E., Otten, U., Agid, Y., and Thoenen, H. 1979. Nerve growth factor (NGF) in the rat CNS: absence of specific retrograde axonal transport and tyrosine hydroxylase induction in locus coeruleus and substantia nigra. Brain Res. 168:473–483.Google Scholar
  2. 2.
    Seiler, M., and Schwab, M. E. 1984. Specific retrograde transport of nerve growth factor (NGF) from neocortex to nucleus basalis in the rat. Brain Res. 300:33–39.Google Scholar
  3. 3.
    Crutcher, K. A., and Collins, F. 1982. In vitro evidence for two distinct hippocampal growth factors: basis of neuronal activity? Science 217:67–68.Google Scholar
  4. 4.
    Ayer-Lelievre, C. S., Ebendal, T., Olson, L., and Seiger, A. 1983. Localization of nerve growth factor-like immunoreactivity in rat nervous tissue. Med. Biol. 61:296–304.Google Scholar
  5. 5.
    Shelton, D. L., and Reichardt, L. F. 1984. Expression of the beta-nerve growth factor gene correlates with the density of sympathetic innervation in effector organs. Proc. Natl. Acad. Sci. USA 81:7951–7955.Google Scholar
  6. 6.
    Korsching, S., Auburger, G., Heumann, R., Scott, J., and Thoenen, H. 1985. Levels of nerve growth factor and its mRNA in the central nervous system of the rat correlate with cholinergic innervation. EMBO J. 4:1389–1394.Google Scholar
  7. 7.
    Whittemore, S. R., Ebendal, T., Larkfors, L., Olson, L., Seiger, A., Stromberg, I., and Persson, H. 1986. Developmental and regional expression of beta nerve growth factor messenger RNA and protein in the rat central nervous system. Proc. Natl. Acad. Sci. USA 83:817–821.Google Scholar
  8. 8.
    Mesulam, M. M., Mufson, E. J., Wainer, B. H., and Levey, A. I. 1983. Central cholinergic pathways in the rat: an overview based on an alternative nomenclature (Ch1–Ch6). Neuroscience 10:1185–1201.Google Scholar
  9. 9.
    Mobley, W. C., Rutkowski, J. L., Tennekoon, G. I., Buchanan, K., and Johnston, M. V. 1985. Choline acetyltransferase activity in striatum of neonatal rats increased by nerve growth factor. Science 229:284–287.Google Scholar
  10. 10.
    Mobley, W. C., Rutkowski, J. L., Tennekoon, G. I., Gemski, J., Buchanan, K., and Johnston, M. V. 1986. Nerve Growth Factor increases choline acetyltransferase in developing basal forebrain neurons. Mol. Brain Res. 1:53–62.Google Scholar
  11. 11.
    Ghahn, H., Hefti, F., Heumann, R., Schwab, M., and Thoenen, H. 1983. NGF-mediated increase of choline acetyltransferase (ChAT) in the neonatal forebrain: evidence for a physiological role of NGF in the brain? Devel. Brain Res. 9:45–52.Google Scholar
  12. 12.
    Hefti, F. 1986. Nerve Growth Factor promotes survival of septal cholinergic neurons after fimbrial transections. J. Neurosci. 6:2155–2162.Google Scholar
  13. 13.
    Sutter, A., Riopelle, R. J., Harris-Warrick, R. M., and Shooter, E. M. 1979. Nerve growth factor receptors. J. Biol. Chem. 254:5972–5982.Google Scholar
  14. 14.
    Richardson, P. M., Verge Issa, V. M. K., and Riopelle, R. J. 1986. Distribution of neuronal receptors for nerve growth factor in the rat. J. Neurosci. 6:2312–2321.Google Scholar
  15. 15.
    Riopelle, R. J., Klearman, M., and Sutter, A. 1980. Nerve growth factor receptors: analysis of the interaction of beta-NGF with membranes of chick embryo dorsal root ganglia. Brain Res. 199:63–77.Google Scholar
  16. 16.
    Rexed, B. 1954. A cytoarchitectonic atlas of the spinal cord in the cat. J. Comp. Neurol. 100:297–379.Google Scholar
  17. 17.
    Puma, P., Buxser, S. E., Watson, L., Kelleher, D. J., and Johnson, G. L. 1983. Purification of the receptor for nerve growth factor from A875 melanoma cells by affinity chromatography. J. Biol. Chem. 258:3370–3375.Google Scholar
  18. 18.
    Riopelle, R. J., Haliotis, T., and Roder, J. C. 1983. Nerve growth factor receptors of human tumors of neural crest origin: characterization of binding site heterogeneity and alteration by theophylline. Cancer Res. 43:5184–5189.Google Scholar
  19. 19.
    Landreth, G. E., and Shooter, E. M. 1980. Nerve growth factor receptors on PC12 cells: ligand induced conversion from low-to-high affinity states. Proc. Natl. Acad. Sci. USA 77:4751–4755.Google Scholar
  20. 20.
    Hosang, M., and Shooter, E. M. 1985. Molecular characteristics of nerve growth factor receptors on PC12 cells. J. Biol. Chem. 260:655–662.Google Scholar
  21. 21.
    Ross, A. H., Grob, P., Bothwell, M., Elder, D. E. Ernst, C. S., Marano, N., Ghrist, B. F. D., Stemp, C. C., Herlyn, M., Atkinson, B., and Koprowski, H. 1984. Characterization of nerve growth factor receptor in neural crest tumors using monoclonal antibodies. Proc. Natl. Acad. Sci. USA 81:6681–6685.Google Scholar
  22. 22.
    Goedert, M., Fine, A., Hunt, S. P., and Ullrich, A. 1986. Nerve Growth Factor mRNA in peripheral and central rat tissues in human central nervous system: lesion effect in the rat brain and levels in Alzheimer disease. Mol. Brain Res. 1:85–92.Google Scholar
  23. 23.
    Johnson, E. M. J., and Yip, H. K. 1985. Central nervous system and peripheral nerve growth factor provide trophic support critical to mature sensory neuronal survival. Nature 314:751–752.Google Scholar

Copyright information

© Plenum Publishing Corporation 1987

Authors and Affiliations

  • R. J. Riopelle
    • 1
  • P. M. Richardson
    • 2
  • V. M. K. Verge
    • 2
  1. 1.Queen's University at KingstonKingstonCanada
  2. 2.McGill University at MontrealCanada

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