Neurochemical Research

, Volume 1, Issue 4, pp 385–402 | Cite as

Biochemical characteristics of C-6 glial cells

  • Antonia Vernadakis
  • Rael Nidess
Original Articles


C-6 glial cells were studied in culture with respect to morphological and biochemical changes under several experimental conditions. Doubling time was 33 hr for cells plated at either 0.5 or 1.0×106 cells per flask. Markedly reduced cell growth and astrocyte-like appearance were observed following dibutyryl cyclic AMP (DBcAMP) treatment. An inverse relationship between cell density and DNA, RNA, and protein content per cell was observed. AChE and BuChE activities were also inversely related to cell density, and treatment with DBcAMP increased enzyme activity, but did not alter the cell density relationship. Uptake of3H-norepinephrine also decreased with increasing cell density. In DBcAMP-treated cells,3H-NE uptake was markedly lower than in nontreated controls, and cortisol treatment decreased the uptake of3H-NE in DBcAMP-treated cells further still. We interpreted the foregoing changes to indicate that cellular activity is cell density-dependent.


Cortisol Cell Density Glial Cell Inverse Relationship Doubling Time 
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  1. 1.
    Edström, A., Kanje, M., andWalum, A. (1974) Effects of dibutyryl cyclic AMP and prostaglandin E1 on cultured human glioma cells.Exp. Cell Res. 85, 217–223.Google Scholar
  2. 2.
    MacIntyre, E.H., Wintersgill, C.J., Perkins, J.P., andVatter, A.E. (1972). The responses in culture of human tumour astrocytes and neuroblasts toN 6,O 2′-dibutyryl adenosine 3′,5′-monophosphoric acid.J. Cell Sci. 11, 639–667.Google Scholar
  3. 3.
    Vernadakis, A., Nidess, R., Timiras, M.L., andSchlesinger, R. (1976) Responsiveness of acetylcholinesterase and butyrylcholinesterase activities in neural cells to age and cell density in culture.Exp. Cell Res. 97, 453–457.Google Scholar
  4. 4.
    Giacobini, E. (1964) Metabolic relations between glia and neurons studied in single cells. In: Cohen, M.M. and Snider, R.S. (eds.),Morphological and Biochemical Correlates of Neural Activity, Harper, New York, pp. 15–38.Google Scholar
  5. 5.
    Gibson, D.A., Reichlin, S., andVernadakis, A. (1974)3H-uridine uptake and incorporation into RNA in the C-6 glial cells following dibutyryl cyclic AMP treatment.Brain Res. 81, 354–360.Google Scholar
  6. 6.
    Ellman, G.L., Courtney, K.L., Andres, V., andFeatherstone, R.M. (1961) A new and rapid colorimetric determination of acetylcholinesterase activity.Biochem. Pharmacol. 7, 88–95.Google Scholar
  7. 7.
    Bayliss, B.J., andTodrick, A. (1956) The use of a selective acetylcholinesterase inhibitor in the estimation of pseudocholinesterase activity in the rat brain.Biochem. J. 62, 62–67.Google Scholar
  8. 8.
    Schmidt, G., andThannhauser, S.J. (1945) Method for the determination of desoxyribonucleic acid, ribonucleic acid, and phosphoproteins in animal tissues.J. Biol. Chem. 161, 83–89.Google Scholar
  9. 9.
    Burton, K. (1956) A study of the conditions and mechanism of diphenylamine reaction for colorimetric estimation of deoxyribonucleic acid.Biochem. J. 62, 315–323.Google Scholar
  10. 10.
    Geel, S., andTimiras, P.S. (1967) The influence of neonatal hypothyroidism and of thyroxine on the ribonucleic acid and deoxyribonucleic acid concentrations of rat cerebral cortex.Brain Res. 4, 1–15.Google Scholar
  11. 11.
    Lowry, O.H., Rosebrough, N.J., Farr, A.L., andRandall, R.J. (1951) Protein measurement with the Folin phenol reagent.J. Biol. Chem. 193, 265–275.Google Scholar
  12. 12.
    Kellog, C., Vernadakis, A., andRutledge, C.L. (1971) Uptake and metabolism of3H-norepinephrine in the cerebral hemispheres of chick embryos.J. Neurochem. 18, 1931–1938.Google Scholar
  13. 13.
    Vernadakis, A. (1973) Uptake of3H-norepinephrine in the cerebral hemispheres and cerebellum of the chicken throughout the lifespan.Mech. Ageing Dev. 2, 371–379.Google Scholar
  14. 14.
    Vernadakis, A. (1974) Neurotransmission: A proposed mechanism of steroid hormones in the regulation of brain function. In: Hatootani, N. (ed.),Psychoneuroendocrinology S. Karger, Basel, pp. 251–258.Google Scholar
  15. 15.
    Vernadakis, A. (1973) Changes in nucleic acid content and butyrylcholinesterase activity in CNS structures during the life span of the chicken.J. Gerontol. 28, 281–286.Google Scholar
  16. 16.
    Iversen, L.L., andSalt, P.J. (1970) Inhibition of catecholamine uptake2 by steroids in the isolated rat heart.Br. J. Pharmacol. 40, 528–530.Google Scholar
  17. 17.
    Pfeiffer, S.E., Herschman, H.R., Lightbody, J., andSato, G. (1970) Synthesis by a clonal line of rat glial cells of a protein unique to the nervous system.J. Cell Physiol. 75, 329–339.Google Scholar
  18. 18.
    Levine, E.M., Becker, Y., Boone, C.W., andEagle, H. (1965) Contact inhibition, macromolecular synthesis, and polyribosomes in cultured human diploid fibroblasts.Proc. Nat. Acad. Sci. U.S.A. 53, 350–356.Google Scholar
  19. 19.
    Levine, E.M., Jeny, D.Y., andChang, Y. (1974) Contact inhibition, polyribosomes, and cell surface membranes in cultured mammalian cells,J. Cell Physiol. 84, 349–363.Google Scholar
  20. 20.
    Warmsley, A.M.H., andPasternak, A. (1970) The use of conventional and zonal centrifugation to study the life cycle of mammalian cells. Phospholipid and macromolecular synthesis in neoplastic mast cells.Biochem. J. 119, 493–499.Google Scholar
  21. 21.
    Prasad, K.N. (1975) Differentiation of neuroblastoma cells in culture.Biol. Rev. 50, 129–265.Google Scholar
  22. 22.
    Stefanovic, V., andMandel, P. (1975) Ecto-enzymes of the nervous system cells in tissue culture. 5th International Meeting of the International Society for Neurochemistry, September 2–6, 1975, Barcelona, Spain.Google Scholar
  23. 23.
    Akeson, R., andHerschman, H.R. (1974) Modulation of cell-surface of a murine neuroblastoma.Proc. Nat. Acad. Sci. U.S.A. 71, 187–191.Google Scholar
  24. 24.
    Erkell, L.J. (1975) Membrane reorganization by mouse neuroblastoma C-1300 differentiation. 5th International Meeting of the International Society for Neurochemistry, September 2–6, 1975, Barcelona, Spain.Google Scholar
  25. 25.
    Truding, K., andMorell, P. (1974) Comparison of surface membranes isolated from cultured murine neuroblastoma cells in the differentiated or undifferentiated state.J. Biol. Chem. 249, 3973–3982.Google Scholar
  26. 26.
    Fritz, A.H., andHamburger, A. (1971) Glial cell function: Uptake of transmitter substances.Proc. Nat. Acad. Sci. U.S.A. 68, 2686–2690.Google Scholar
  27. 27.
    Woodbury, D.M. (1958) Relation between the adrenal cortex and the central nervous system.Pharmacol. Rev. 10, 275–357.Google Scholar
  28. 28.
    Vernadakis, A., andWoodbury, D.M. (1971) Effects of cortisol on maturation of the central nervous system. In: Ford, D.H. (ed.),Influence of Hormones on the Nervous System, S. Karger, Basel, pp. 85–97.Google Scholar
  29. 29.
    Vernadakis, A., andWoodbury, D.M. (1965) Cellular and extracellular spaces in developing rat brain. Radioactive uptake studies with chloride and insulin.Arch. Neurol. 12, 284–293.Google Scholar
  30. 30.
    Weiss, B., andStrada, S.J. (1973) Adenosine 3′,5′-monophosphate during fetal and postnatal development. In:Boreús, L. (ed.),Fetal Pharmacology, Raven Press, New York, pp. 205–235.Google Scholar

Copyright information

© Plenum Publishing Corporation 1976

Authors and Affiliations

  • Antonia Vernadakis
    • 1
  • Rael Nidess
    • 1
  1. 1.Departments of Psychiatry and PharmacologyUniversity of Colorado School of MedicineDenver

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