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Effect of ferric nitrilotriacetate on predominately cortical glial cell cultures

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Abstract

Cultured glial cells were exposed to ferric nitrilotriacetate (Fe-NTA) at varying concentrations. Studies of the exposed glial cells were performed at days 29 and 36 post-conceptional age (culture days 8 and 15). In addition to morphologic studies, biochemical assays including [3H]-flunitrazepam (FLU) specific binding, Ro5-4864-displaceable3H-FLU binding, and protein determinations were performed. At day 29 post-conceptional age, significant decreases in3H-FLU specific binding, Ro5-4864-displaceable3H-FLU binding, and protein determinations were discernible only in the presence of 100 μM Fe-NTA. At day 36 post-conceptional age3H-FLU specific binding was significantly decreased at 20, 60, and 100 μM Fe-NTA concentrations, while Ro5-4864-displaceable3H-FLU binding and protein determinations were significantly reduced at 60 and 100 μM Fe-NTA concentrations. The effects of Fe-NTA exposure appear to be both concentration and duration-of-exposure related. When compared to previously reported neuronal cell culture, studies utilizing3H-FLU specific binding, Ro5-4864-displaceable3H-FLU binding, and protein determinations, glial cells appear to be significantly more resistant to chelated iron exposure.

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Authors and Affiliations

  1. Division of Pediatric Neurology, University of Minnesota Medical School, Minneapolis, Minnesota

    Kenneth F. Swaiman & Valynda L. Machen

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  1. Kenneth F. Swaiman
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  2. Valynda L. Machen
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Swaiman, K.F., Machen, V.L. Effect of ferric nitrilotriacetate on predominately cortical glial cell cultures. Neurochem Res 15, 501–505 (1990). https://doi.org/10.1007/BF00966207

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