Neurochemical Research

, Volume 11, Issue 9, pp 1289–1301 | Cite as

Preparation of a cell-free extract from rat brain which can initiate protein synthesis in vitro

  • James W. Cosgrove
  • Stanley I. Rapoport
Original Articles

Abstract

A cell-free protein synthesis system, derived from brains of 3 mo-old male Fischer-344 rats, has been characterized. The optimum conditions for amino acid incorporation in the system were 5 mM magnesium ion and 200 mM potassium ion. Incorporation depended on the addition of ATP, GTP, and an enegy-generating system, and was sensitive to addition of the drugs aurintricarboxylic acid and sodium fluoride, inhibitors of initiation of protein synthesis. Both 40S and 80S initiation complexes were labeled in vitro, using [35S]methionine. Such labeling was sensitive to the protein synthesis inhibitors, aurintricarboxylic acid and sodium fluoride. The system, which can initiate protein synthesis, should be of use for examining mechanisms which underlie alterations in rat brain protein synthesis induced by various treatments.

Keywords

Protein Synthesis Protein Synthesis Inhibitor Sodium Fluoride Initiation Complex Brain Protein 

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Copyright information

© Plenum Publishing Corporation 1986

Authors and Affiliations

  • James W. Cosgrove
    • 1
  • Stanley I. Rapoport
    • 1
  1. 1.Laboratory of NeurosciencesNational Institute on Aging NIHBethesda

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