Neurochemical Research

, Volume 2, Issue 5, pp 581–593 | Cite as

Calcium content and binding in synaptosomal subfractions during chronic morphine treatment

  • David H. Ross
Article

Abstract

Chronic exposure to morphine in mice produced an increase in Ca2+ content of synaptosomes, synaptic plasma membranes (SPM), and synaptic vesicles. Ca2+ binding capacity was significantly reduced in tolerant SPM fractions. Naloxone significantly reversed the increased calcium content and reduced binding capacity of SPM when administered to 72-h-treated mice. Scatchard analysis of binding curves reveals three distinct classes of Ca2+ binding sites. During tolerance, the high- and low-affinity sites exhibit a reduced capacity to bind calcium, which may be reversed by in vivo and in vitro administration of naloxone. The increase in SPM and synaptic vesicle calcium content may reflect adaptive changes in the cell membrane during tolerance development, which may contribute to changes in neurotransmitter and second messenger function.

Keywords

Calcium Morphine Naloxone Binding Capacity Chronic Exposure 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Publishing Corporation 1977

Authors and Affiliations

  • David H. Ross
    • 1
  1. 1.Departments of Pharmacology and PsychiatryThe University of Texas Health Science CenterSan Antonio

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