Neurochemical Research

, Volume 11, Issue 11, pp 1565–1569 | Cite as

Azidomorphine is an agonist of high-affinity opioid receptor binding sites

  • Katalin Horváth
  • Mária Wollemann
Original Articles
  • 17 Downloads

Abstract

Azidomorphine at low concentration (10−9 M) inhibits the high-affinity binding site of labeled naloxone in rat brain membrane preparations. In the presence of Na+ and guanine nucleotides the displacement curves of azidomorphine are increased toward high concentrations, whereas Mg2+ ions decrease the IC50 values; This demonstrates the agonist behavior of azidomorphine in binding experiments. When compared with morphine, azidomorphine displayed five-fold lower IC50 values. Based on the presented results, azidomorphine appears to be a good candidate for photoaffinity labeling of opiate receptors.

Keywords

Morphine Guanine Naloxone Receptor Binding Opioid Receptor 

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Copyright information

© Plenum Publishing Corporation 1986

Authors and Affiliations

  • Katalin Horváth
    • 1
  • Mária Wollemann
    • 1
  1. 1.Institute of Biochemistry Biological ResearchCenter of Hungarian Academy of SciencesSzegedHungary

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