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Neurochemical Research

, Volume 15, Issue 10, pp 981–985 | Cite as

Lipid order and composition of synaptic membranes in experimental diabetes mellitus

  • Arshag D. Mooradian
  • Frank Dickerson
  • Thomas L. Smith
Original Articles

Abstract

The effect of diabetes in rats on lipid composition and order of synaptosomal membranes (SM) was determined in streptozotocin-induced diabetic rats after 6 weeks of chronic hyperglycemia. The cholesterol content was slightly, but not significantly, higher in diabetic SM (0.287±0.042 vs. 0.209±0.061 μmol/mg protein). The phospholipid concentration in diabetic SM was significantly increased (0.515±0.042 vs. 0.305±0.041 μmol/mg protein;P<0.005). Neither the molar ratios of cholesterol to phospholipids in the SM nor the fatty acid composition of the SM was significantly altered with diabetes. Diabetes did not affect membrane order or the thermotropic transition temperature of the SM as determined fluorometrically. On the other hand, the SM of diabetic rats had significantly increased concentration of lipid peroxidation products, namely conjugated dienes (the calculated O.D./μmol phospholipids was 11.56±1.83 in controls and 19.95 ±4.1 in diabetic ratsP<0.01). Despite the accumulation of lipid peroxidation byproducts in SM of diabetic rats the overall membrane order and the cholesterol to phospholipid molar ratio do not appear to be significantly altered.

Key Words

Synaptosome lipid order lipid composition conjugated dienes diabetes 

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Copyright information

© Plenum Publishing Corporation 1990

Authors and Affiliations

  • Arshag D. Mooradian
    • 1
    • 2
  • Frank Dickerson
    • 3
  • Thomas L. Smith
    • 4
  1. 1.The Medical Service, Department of MedicineUniversity of Arizona College of MedicineTucson
  2. 2.The Research Service, Department of MedicineUniversity of Arizona College of MedicineTucson
  3. 3.The Research Service Tucson VA Medical centerUniversity of Arizona College of MedicineTucson
  4. 4.The Research Service, Department of PharmacologyUniversity of Arizona College of MedicineTucson

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