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Lipid fluidity and composition of the erythrocyte membrane from healthy dogs and labrador retrievers with hereditary muscular dystrophy

Neurochemical Research volume 16, pages 129–135 (1991)Cite this article

Abstract

Erythrocyte membranes and their liposomes were prepared from clinically normal dogs and Labrador retrievers with hereditary muscular dystrophy. The “static” and “dynamic” components of fluidity of each membrane were then assessed by steady-state fluorescence polarization techniques using limiting hindered fluorescence anisotropy and order parameter values of 1,6-diphenyl-1,3,5-hexatriene (DPH) and fluorescence anisotropy values ofdl-2-(9-anthroyl)-stearic acid anddl-12-(9-anthroyl)-stearic acid, respectively. Membrane lipids were extracted and analyzed by thin-layer chromatography and gas chromatography. The results of these studies demonstrated that the lipid fluidity of erythrocyte membranes, and their liposomes, prepared from dystrophic dogs were found to possess significantly lower “static and dynamic components of fluidity” than control counterparts. Analysis of the composition of membranes from dystrophic dogs revealed a higher ratio of saturated fatty acyl chain/unsaturated chains (w/w) and lower double-bond index. Alterations in the fatty acid composition such as decrease in levels of linoleic (18:2) and arachidonic (20:4) acids and increase in palmitic (16:0) and stearic (18:0) acids were also observed in the membranes of dystrophic animals. These associated fatty acyl alterations could explain, at least in part, the differences in membrane fluidity between dystrophic and control dogs.

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Author notes

  1. Vijay Thukral

    Present address: Department of Neurology and Neurosciences, Tuft School of Medicine, 02111, Boston, MA

Affiliations

  1. Neuromuscular Laboratories of Scott-Ritchey Research Program, College of Veterinary Medicine, Auburn University, 36849, AL

    Jagjivan R. Mehta, Kyle G. Braund & Vijay Thukral

  2. Department of Veterinary Pathology, College of Veterinary Medicine, Washington State University, 99163, Pul'man, Washington

    Gerald A. Hegreberg

Authors
  1. Jagjivan R. Mehta
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  2. Kyle G. Braund
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  3. Gerald A. Hegreberg
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Mehta, J.R., Braund, K.G., Hegreberg, G.A. et al. Lipid fluidity and composition of the erythrocyte membrane from healthy dogs and labrador retrievers with hereditary muscular dystrophy. Neurochem Res 16, 129–135 (1991). https://doi.org/10.1007/BF00965700

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Key Words

  • Erythrocytes
  • membrane fluidity
  • muscular dystrophy
  • lipid composition
  • fluorescence polarization