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Neurochemical Research

, Volume 16, Issue 2, pp 123–128 | Cite as

The phylogenic expression of plasmolipin in the vertebrate nervous system

  • Victor S. Sapirstein
  • Charles E. Nolan
  • Itzhak Fischer
  • Elizabeth Cochary
  • Susana Blau
  • Cheryl J. Flynn
Original Articles

Abstract

Plasmolipin is a plasma membrane proteolipid is a major myelin membrane component (Cochary et al., 1990). In this study we report the phylogenic expression of plasmolipin in the vertebrate nervous system. Using Western blot analysis with polyclonal antibodies, we have analyzed membrane fractions, including myelin, from elasmobranchs, teleosts, amphibians, reptiles, birds and mammals. On the basis of immune detection, plasmolipin appears to be restricted to the mammalian nervous system. Comparison of the central and peripheral nervous systems of mammals showed only minor differences in the level of plasmolipin in these two regions. Within mammals, little quantitative differences were observed when rat, human and bovine membrane fractions were compared. The late evolutionary expression of plasmolipin which results in its restriction to mammals makes it unique among the (major) myelin proteins. The potential physiologic significance of these data are discussed.

Key Words

Plasma membrane proteolipid myelin evolution mammalian brain 

Abbreviations

EDTA

Ethylene diamine N.,N′N′ tetracetic acid

EGTA

Ethylene glycol bis-(B-Aminoethyl Ether) N,,N′N′ tetracetic acid

MES

([N-Morpholino] ethane sulfonic acid) DCCD, N′, Dicyclohexyl carbodiimide

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Copyright information

© Plenum Publishing Corporation 1991

Authors and Affiliations

  • Victor S. Sapirstein
    • 1
    • 2
  • Charles E. Nolan
    • 1
    • 2
  • Itzhak Fischer
    • 3
    • 4
  • Elizabeth Cochary
    • 3
    • 4
  • Susana Blau
    • 1
    • 2
  • Cheryl J. Flynn
    • 1
    • 2
  1. 1.The Division of NeurobiologyThe Nathan Kline InstituteOrangeburg
  2. 2.Department of PsychiatryNew York University School of MedicineNew York
  3. 3.The Department of BiochemistryThe Eunice Kennedy Shriver CenterWaltham
  4. 4.Department of NeurologyHarvard Medical SchoolBoston

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