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Neurochemical Research

, Volume 9, Issue 6, pp 849–862 | Cite as

Substrate specificity and distribution of acid β-galactosidase activities in seizure-susceptible and non-susceptible strains of mice

  • D. F. Wheeler
  • H. S. Bachelard
Original Articles
  • 39 Downloads

Abstract

The properties and distribution of β-galactosidase were studied in the mouse brain using the artificial substrate methylumbelliferyl-β-galactoside. Enzyme activities were compared between an audiogenic seizure-susceptible mouse strain (DBA/2) and three non-susceptible strains of mice (BALB/c, C3H/He and Swiss A2G). At all ages, DBA/2 mice have significantly lower β-galactosidase activity compared with the three other mouse strains: this is attributed to the different alleles present at the Bgs locus. The low activity of β-galactosidase is also evident when the natural substrate GMI-ganglioside is hydrolyzed. In contrast to this low GMI-ganglioside-β-galactosidase activity, there is no difference in the activity of the second form of acid β-galactosidase, galactosylceramidase, in DBA/2 mice at 7 and 14 days. However, at 21 and 28 days the activity is significantly lower in DBA/2 mice compared with the other strains of mice. These results on β-galactosidase activity in the brain of seizure-susceptible and non-susceptible mice are discussed in relation to published levels of GMI-ganglioside and galactosylceramide present in the developing mouse brain.

Keywords

Enzyme Activity Substrate Specificity Mouse Brain Mouse Strain Natural Substrate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Publishing Corporation 1984

Authors and Affiliations

  • D. F. Wheeler
    • 1
  • H. S. Bachelard
    • 1
  1. 1.Department of BiochemistrySt. Thomas's Hospital Medical SchoolLondonUK

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