Neurochemical Research

, Volume 9, Issue 6, pp 751–757 | Cite as

Kinetic properties of glutaminase from cerebral cortex

  • H. F. Bradford
  • H. K. Ward
  • M. Sandberg
Original Articles


The rates of phosphate activated glutaminase activity in finely homogenised cerebral cortex and synaptosomes were measured. Activity was 25–50% higher at pH 7.0 than at pH 8.0. Glutamate inhibited activity with a Ki of 2–3 mM while aspartate had little effect. Calcium (1 mM) activated the enzyme but magnesium was without action. The pH profiles of the effects of these modulators of glutaminase activity in these finely ground preparations showed that all agents were more effective at pH 7.0 than at pH 8.0.


Calcium Phosphate Magnesium Aspartate Glutamate 
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  1. 1.
    Bradford, H. F., Ward, H. K., andThomas, A. J. 1978. Glutamine—a major substrate for nerve endings. J. Neurochem. 30:1453–1459.PubMedGoogle Scholar
  2. 2.
    Hamberger, A. C., Chiang, G. H., Nylén, E. S., Scheff, S. W., andCotman, C. W. 1979. Glutamate as a CNS transmitter. I. Evaluation of glucose and glutamine as precursors for the synthesis of preferentially released glutamate. Brain Res. 168:513–530.PubMedGoogle Scholar
  3. 3.
    Reubi, J. C., Van Den Berg, C. J., andCuénod, M. 1978. Glutamine as precursor for the GABA and glutamate transmitter pools. Neurosci. Lett. 10:171–174.Google Scholar
  4. 4.
    Tapia, R., andGonzález, R. M. 1978. Glutamine and glutamate as precursors of the releasable pool of GABA in brain cortex slices. Neurosci. Lett. 10:165–169.Google Scholar
  5. 5.
    Kvamme, E., andLenda, K. 1982. Regulation of glutaminase by exogenous glutamate, ammonia and 2-oxoglutarate in synaptosomal enriched preparation from rat brain. Neurochem. Res. 7:667–678.PubMedGoogle Scholar
  6. 6.
    Benjamin, A. M. 1981. Control of glutaminase activity in rat brain cortex in vitro: Influence of glutamate, phosphate, ammonium, calcium and hydrogen ions. Brain Res. 208:363–377.PubMedGoogle Scholar
  7. 7.
    Bradford, H. F., andWard, H. K. 1976. On glutaminase activity in mammalian synaptosomes. Brain Res. 110:115–125.PubMedGoogle Scholar
  8. 8.
    Weil-Malherbe, H. 1969 Activators and inhibitors of brain glutaminase. J. Neurochem. 16:855–864.PubMedGoogle Scholar
  9. 9.
    Kvamme, E., Svenneby, G., andTorgner, I. AA. 1983 Calcium stimulation of glutamine hydrolysis in synaptosomes from rat brain. Neurochem. Res. 8:23–36.Google Scholar
  10. 10.
    Bradford, H. F., Bennet G. W., andThomas, A. J. 1973. Depolarizing stimuli and the release of physiologically active amino acids from suspensions of mammalian synaptosomes. J. Neurochem. 21:495–505.PubMedGoogle Scholar
  11. 11.
    Lowry, O. H., Rosebrough, N. J., Farr, A. L., andRandall, R. J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265–275.PubMedGoogle Scholar
  12. 12.
    Kvamme, E., andLenda, K. 1981. Evidence for compartmentalization of glutamate in rat brain synaptosomes using the glutamate sensitivity of phosphate-activated glutaminase as a functional test. Neurosci. Lett. 25:193–198.PubMedGoogle Scholar
  13. 13.
    Edsall, J. T., andWyman, J. 1958. Page 452,Biophysical Chemistry Vol I Academic Press, New York.Google Scholar

Copyright information

© Plenum Publishing Corporation 1984

Authors and Affiliations

  • H. F. Bradford
    • 1
  • H. K. Ward
    • 1
  • M. Sandberg
    • 1
  1. 1.Department of BiochemistryImperial CollegeLondon

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