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NAD+-dependent formation ofγ-aminobutyrate (GABA) from glutamate

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Abstract

Mitochondria and nuclei of various tissues, including brain and liver, are capable of producingγ-aminobutyrate (GABA) fromL-glutamate, but poorly, if at all, fromD-glutamate. The amino nitrogen of glutamate is found in the reaction product. The enzymes responsible for GABA formation were solubilized from crude liver cell nuclei by Triton X-100. The reaction is NAD+ dependent Oxygen, FMN, Mg2+, and pyridoxalphosphate enhanced GABA formation. NADP+, coenzyme A, ornithine, 2-oxoglutarate, and aminooxyacetic acid, among others, inhibited the formation of GABA. On the basis of the available information the reaction sequence, is formulated tentatively as follows:

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  1. Arbeitsgruppe Neurochemie, Max-Planck-Institut für Hirnforschung, Frankfurt/M, G.F.R.

    Nikolaus Seiler & Gernot Wagner

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  1. Nikolaus Seiler
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  2. Gernot Wagner
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Seiler, N., Wagner, G. NAD+-dependent formation ofγ-aminobutyrate (GABA) from glutamate. Neurochem Res 1, 113–131 (1976). https://doi.org/10.1007/BF00965636

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