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Isotopic labeling of synaptosomes that accumulate choline and the effect of narcotic drugs

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Abstract

Subcellular studies of choline uptake of rat striatum indicated a correspondence between the Na+-dependent uptake and choline acetyltransferase (ChAc), whereas there was a lack of correspondence between the Na+-independent uptake and ChAc. Subcellular studies also showed a correspondence between the Na+-dependent uptake and hemicholinium-3 inhibition, and more important, particles that accumulate choline were shown to consist of at least two subcellular populations. A comparison was made of kinetic data from three areas of the rat brain: corpus striatum, cerebral cortex, and hypothalamus. Taken together, our data on choline uptake give added support to the idea that the Na+-dependent choline transport is concentrated in the striatum and specifically related to cholinergic nerve endings. Morphine and methadone in vitro inhibited the Na+-dependent choline uptake. In vivo morphine induced a significant lowering of theV max in the rat cerebral cortex, but not in the striatum. This finding is consistent with the known action of morphine on acetylcholine turnover.

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Authors and Affiliations

  1. Rockland Research Institute, Research Institute for Neurochemistry and Drug Addiction, 10035, Ward's Island, New York

    James P. Hudick, Isabel J. Wajda & Abel Lajtha

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  1. James P. Hudick
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  2. Isabel J. Wajda
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  3. Abel Lajtha
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Hudick, J.P., Wajda, I.J. & Lajtha, A. Isotopic labeling of synaptosomes that accumulate choline and the effect of narcotic drugs. Neurochem Res 1, 609–625 (1976). https://doi.org/10.1007/BF00965602

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