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Diaminobutyric acid: A tool for discriminating between carrier-mediated and non-carrier-mediated release of GABA from synaptosomes?

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Abstract

The carrier-mediated transport of GABA in rat brain synaptosomes was strongly and permanently inhibited byl-2,4-diaminobutyric acid (DAB). In order to discriminate between carrier-mediated and non-carrier-mediated release of [3H]GABA, synaptosomes prelabeled with 0.5 μM [3H]GABA in the presence of 100 μM DAB, or with 0.2 μM [3H]GABA without DAB, were superfused in conditions stimulating the release of [3H]GABA. Only the release elicited by unlabeled GABA or DAB (by homo- and heteroexchange, respectively) was strongly inhibited in DAB-pretreated synaptosomes. The spontaneous release and the release induced by 56 mM KCl in the presence of CaCl2, by the ionophore A23187, by ouabain, by lack of K+, or by purified black widow spider toxin were unaffected or only barely decreased in DAB-treated synaptosomes, and therefore do not seem to be mediated by the DAB-blocked GABA carrier.

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Authors and Affiliations

  1. Laboratorio di Biologia Cellulare, CNR, Via Romagnosi 18/A, Rome, Italy

    Giulio Levi & Graziella Rusca

  2. Istituto di Farmacologia, Università Cattolica, Rome, Italy

    Maurizio Raiteri

Authors
  1. Giulio Levi
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  2. Graziella Rusca
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  3. Maurizio Raiteri
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Levi, G., Rusca, G. & Raiteri, M. Diaminobutyric acid: A tool for discriminating between carrier-mediated and non-carrier-mediated release of GABA from synaptosomes?. Neurochem Res 1, 581–590 (1976). https://doi.org/10.1007/BF00965600

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  • DOI: https://doi.org/10.1007/BF00965600

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