Reduction of cellular damage induced by cerebral ischemia in rats

Abstract

A model of incomplete cerebral ischemia involving bilateral ligation of the common carotid arteries in rats, was used to examine the potential of a Chinese herbal preparation and of nifedipine to reduce cell damage following cerebral ischemia. The herbal preparation contained ginsengosides and extracts ofPanax notoginseng, Ligusticum chuanxiong Hort.,Carthamus tinctorius L. andSalvia militorrhiza Bge. Histological evidence of cell damage and the formation of peroxidation products were both reduced in rats pretreated with the herbal preparation or with nifedipine. It has been suggested that the free radical reaction is involved in tissue damage, particularly in the pathological neurocyte injury of cerebral ischemia. The results show that in this model of incomplete cerebral ischemia, the degree of lipid peroxidation can be lowered by the pretreatment with Chinese herbs containing ginsengosides or with nifedipine. These drugs maybe beneficial in the treatment of cerebral ischemia in humans.

This is a preview of subscription content, log in to check access.

References

  1. 1.

    Simpson J. A. 1984. Diseases of the nervous system, Pages 627–630in Macleod J. (ed.) Principles and Practice of Medicine, 14th ed. Churchill Livingstone, U. K.

    Google Scholar 

  2. 2.

    Edvinsson L., Brandt L., Andersson K. E., and Bengtsson B. 1979. Effect of calcium antagonist on experimental constriction of human brain vessels. Surg. Neurol. 11:327–330.

    Google Scholar 

  3. 3.

    Shimizn K., 1980. Evidence for greater susceptibility of isolated dog cerebral arteries to Ca++-antagonists than peripheral arteries. Stroke 11:261.

    Google Scholar 

  4. 4.

    Feng Y. P., Sung Y. D., Chen B. T., Ye G. and Zeng G. Y., 1989. Brain energy metabolism of cerebral ischemia mice and the effects of some drugs. Acta Pharmaceutica Sinica 24(2):89–94.

    Google Scholar 

  5. 5.

    Luo Jun, 1987. Effect of Nao Mei Kang on microcirculation and blood pressure in rats. Academic Journal of the First Military Medical College 7(2):151–154.

    Google Scholar 

  6. 6.

    Yagi K., 1976. A simple fluorometric assay for lipoperoxide in blood plasma. Biochem. Med. 15:212–215.

    Google Scholar 

  7. 7.

    Csallany A. S., Guan M. D., Manwaring, J. D., and Addis P. B., 1984. Free malonaldehyde determination in tissues by high-performance liquid chromatography. Anal. Biochem. 142:277–283.

    Google Scholar 

  8. 8.

    Rehncrona S., Smith D. S., Akesson B., Westerberg E., and Siesjo B. K., 1980. Peroxidative changes in brain cortical fatty acids and phospholipids, as characterized during Fe++-and ascorbic acid-stimulated lipid peroxidation in vitro. J. Neurochem. 34:1630–1638.

    Google Scholar 

  9. 9.

    Demopoulos H. B. 1977. Molecular pathology of lipid in CNS membranes. In: Jobsis F. F. ed. Oxygen and physiological function. Professional Information Library. 491–508.

  10. 10.

    Wu K. H., Leung A. W. N., and Cheng C. H. K., 1988. The effect of chronic ethanol ingestion on free radical scavenging enzymes in rat skeletal muscle. In Proceedings of the Society for the Study of Endocrinology, Metabolism and Reproduction, 15.

  11. 11.

    Del Maedtro R. F. 1980. An approach to free radical in medicia and biology. Acta Physiol. Scand 492 (suppl):153–168.

    Google Scholar 

  12. 12.

    Machlin L. J., and Bendich A. 1987. Free radical tissue damage: protective role of antioxidant nutrients. FASEB J. 1:441–445.

    Google Scholar 

  13. 13.

    Yagi K., 1987. Lipid peroxides and human diseases. Chem. Phys. Lipids, 45:337–351.

    Google Scholar 

Download references

Author information

Affiliations

Authors

Additional information

Special issue dedicated to Dr. Lawrence Austin.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Leung, A.W.N., Mo, Z. & Zheng, Y. Reduction of cellular damage induced by cerebral ischemia in rats. Neurochem Res 16, 687–692 (1991). https://doi.org/10.1007/BF00965556

Download citation

Key Words

  • Cerebral ischemia
  • malondialdehyde
  • nifedipine
  • Chinese herbal ginsengosides