Neurochemical Research

, Volume 19, Issue 9, pp 1175–1180 | Cite as

Effect of phenylalanine and its metabolites on ATP diphosphohydrolase activity in synaptosomes from rat cerebral cortex

  • Angela Terezinha De Souza Wyse
  • João José Freitas Sarkis
  • João Sabino Cunha-Filho
  • Marcio Vieira Teixeira
  • Maria Rosa Schetinger
  • Moacir Wajner
  • Clovis Milton Duval Wannmacher
Original Articles


The in vitro effects of phenylalanine and some of its metabolites on ATP diphosphohydrolase (apyrase, EC activity in synaptosomes from rat cerebral cortex were investigated. The enzyme activity in synaptosomes from rats subjected to experimental hyperphenylalaninemia (α-methylphenylalanine plus phenylalanine) was also studied. In the in vitro studies, a biphasic effect of phenylalanine on both enzyme substrates (ATP and ADP) was observed, with maximal inhibition at 2.0 mM and maximal activation at 5.0 mM. Inhibition of the enzyme activity was not due to calcium chelation. Moreover, phenylpyruvate, when compared with phenylalanine showed opposite effects on the enzyme activity, suggesting that phenylalanine and phenylpyruvate bind to two different sites on the enzyme. The other tested phenylalanine metabolites (phenyllactate, phenylacetate and phenylethylamine) had no effect on ATP diphosphohydrolase activity. In addition, we found that ATP diphosphohydrolase activity in synaptosomes from cerebral cortex of rats with chemically induced hyperphenylalaninemia was significantly enhanced by acute or chronic treatment. Since it is conceivable that ATPase-ADPase activities play an important role in neurotransmitter (ATP) metabolism, it is tempting to speculate that our results on the deleterious effects of phenylalanine and phenylpyruvate on ATP diphosphohydrolase activity may be related to the neurological dysfunction characteristics of naturally and chemically induced hyperphenylalaninemia.

Key Words

ATP diphosphohydrolase apyrase phenylketonuria phenylalanine phenylalanine metabolites synaptosomes 


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Copyright information

© Plenum Publishing Corporation 1994

Authors and Affiliations

  • Angela Terezinha De Souza Wyse
    • 1
  • João José Freitas Sarkis
    • 2
  • João Sabino Cunha-Filho
    • 2
  • Marcio Vieira Teixeira
    • 2
  • Maria Rosa Schetinger
    • 2
  • Moacir Wajner
    • 2
  • Clovis Milton Duval Wannmacher
    • 1
  1. 1.Departamento de Clências FisiológicasFundacão Universidade do Rio GrandeRio GrandeBrazil
  2. 2.Departmento de Bloquímica, Instituto de BiociênciasUniversidade Federal do Rio Grande do SulPorto AlegreBrazil

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