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Effects of ATP on calcium binding to synaptic plasma membrane

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Abstract

The release of labeled norepinephrine from preloaded synaptosomes requires the presence of potassium and calcium. ATP-dependent binding of calcium to synaptic plasma membranes (SPM) may provide a means of maintaining the cation in a readily available pool for the triggering of transmitter release. A high Ca-binding capacity was demonstrated in SPM. The Km for calcium is 5.5×10−5 M. The dependence of the system on the gamma phosphate of ATP was demonstrated by an increase in Ca-binding with increasing ATP concentration and by competitive inhibition of binding by ADP and AMP. Magnesium is also required for ATP-dependent Ca-binding. The optimum pH for the Ca binding was 7.0. Pretreatment of SPM with phospholipase A2 lowered the binding capacity. Sulfhydryl groups are also critical for ATP-dependent Ca binding to occur. A model for ATP-dependent Ca-binding was proposed.

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Authors and Affiliations

  1. Sinclair Comparative Medicine Research Farm and Department of Biochemistry, University of Missouri, 65201, Columbia, Missouri

    V. Corpus & A. Y. Sun

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  1. V. Corpus
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  2. A. Y. Sun
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Corpus, V., Sun, A.Y. Effects of ATP on calcium binding to synaptic plasma membrane. Neurochem Res 8, 501–520 (1983). https://doi.org/10.1007/BF00965106

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