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Mn 2+-stimulatedATPase in rat brain

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Abstract

Divalent cation ATPases were prepared from rat brain synaptic vesicles, synaptosomal plasma membranes, and plasma membranes from the brain stem and sciatic nerve and tested for optimal stimulation by Mn2+, Mg2+, or Ca2+. ATPase in the synaptic vesicle subfraction was optimally stimulated by Mn2+. All plasma membrane preparations were optimally stimulated by Mg2+. Separate Mn2+ and Mg2+ ATPases could not be distinguished by either chemical inactivation or substrate preference criteria. Mn2+ stimulated ATPase in the micromolar range and it is suggested that Mn2+ interaction with ATPase may be of physiological and/or toxicological importance by being related to the cellular metabolism of this element.

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References

  1. Bonilla, E., Salazar, E., Villasmil, J. J., andVillalobos 1982. The regional distribution of manganese in the normal human brain. Neurochemical Research 7:221–227.

    PubMed  Google Scholar 

  2. Chandra, S. V., andShukla, G. S. 1981. Concentration of striatal catecholamines in rats given manganese chloride through drinking water. J. Neurochem. 36:683–687.

    PubMed  Google Scholar 

  3. Cotzias, G. C. 1958. Manganese in health and disease. Physiol. Rev. 38:503–532.

    PubMed  Google Scholar 

  4. Cotzias, G. C., Papavasiliou, P. S., Ginos, J., Steck, A., andDuby, S. 1971. Metabolic modification of Parkinson's disease and of chronic manganese poisoning. Ann. Rev. Med. 22:305–326.

    PubMed  Google Scholar 

  5. DeVries, G. H., Matthieu, J. M., Beny, M., Chicheportiche, R., Lazdunski, M., andDolivo, M. 1978. Isolation and partial characterization of rat CNS axolemma enriched fractions. Brain Res. 147:339–352.

    PubMed  Google Scholar 

  6. Doherty, J. D., Salem, N., Jr., Lauter, C. J., andTrams, E. G. 1981. Mn2+ and Ca2+ ATPases in lobster axon plasma membranes and their inhibition by pesticides. Comp. Biochem. Physiol. 69C:185–190.

    Google Scholar 

  7. Donaldson, J., St Pierre, T., Minnich, J. L., andBarbeau, A. 1973. Determination of Na2+, K+, Mg2+, Cu2+, Zn2+, and Mn2+ in rat brain regions. Can. J. Biochem. 51:87–92.

    PubMed  Google Scholar 

  8. Edstrom, A., Hanson, M., Prus, K., andWallin, M. 1980. Ca2+ or Mg2+-dependent enzyme ATP hydrolysis associated with microsomal fraction of frog sciatic nerve. J. Neurochem. 35:297–303.

    PubMed  Google Scholar 

  9. Ehringer, H., andHornykiewcz, O. 1960. Verteilung von Noradrenalin und Dopamin (3-hydroxytyramin). Im Gehren des Menschen und ihr Verhalten bei Erkrankungen des Extrapyramidalin System. Klin Wschr. 38:1236–1239.

    PubMed  Google Scholar 

  10. Fiske, C. H., andSubarrow, Y. 1925. The colormetric determination of phosphorous. J. Biol. Chem. 66:375–378.

    Google Scholar 

  11. Hanig, R., andAprison, M. H. 1967. Determination of calcium, copper, iron, magnesium, manganese, potassium, sodium, zinc, and chloride concentrations in several brain areas. Anal. Biochem. 21:169–177.

    PubMed  Google Scholar 

  12. Ito, N., Kosita, M., andTanaka, R. 1982. Actomyosin-like ATPase extracted from plain synaptic vesicles fractions. Neurochem. International 4:247–258.

    Google Scholar 

  13. Javors, M. A., Bowden, C. L., andRoss, D. H. 1981. Kinetic characterization of Ca2+ transport in synaptic membranes. J. Neurochem. 37:381–387.

    PubMed  Google Scholar 

  14. Jones, D. H., andMatus, A. I. 1974. Isolation of synaptic plasma membrane from brain by combined flotation-sedimentation density gradient centrifugation. Biochem. Biophys. Acta 356:276–287.

    PubMed  Google Scholar 

  15. Kadota, K., andKadota, T. 1973. Isolation of coated vesicles, plain synaptic vesicles, and flocculent material from a crude synaptosome fraction of guinea pig whole brain. J. of Cell Biol. 58:135–151.

    Google Scholar 

  16. Mena, I., Marin, O., Fuenzalida, S., andCotzias, G. C. 1967. Chronic manganese poisoning. Clinical picture and manganese turnover. Neurol. 17:128–36.

    Google Scholar 

  17. Michaelson, D. M., andOphir, E. 1980. Sidedness of (Calcium and Magnesium) adenosine triphosphatase of purified Torpedo synaptic vesicles. J. Neurochem. 34:1483–90.

    PubMed  Google Scholar 

  18. Scheuhammer, A. M., andCherian, M. G. 1981. The influence of manganese on the distribution of essential trace elements. Toxicol. Appl. Pharmacol. 61:227–233.

    PubMed  Google Scholar 

  19. Seth, P. K., Musain, R., Mushtag, M., andChandra, S. B. 1977. Effect of manganese on neonatal rat: Manganese concentration and enzymatic alterations in brain. Acta. pharmacol. et toxicol. 40:553–560.

    Google Scholar 

  20. Tanaka, R., Asaga, H., andTakeda, M. 1976. Nucleoside triphosphate and cation requirement for dopamine uptake by plain synaptic vesicles isolated from rat cerebrums. Brain. Res., 115:272–283.

    Google Scholar 

  21. Trams, E. G., andLauter, C. J. 1978. A comparative study of brain Ca2+ ATPases. Comp. Biochem. Physiol. 59B:191–194.

    Google Scholar 

  22. Tsudzuki, T. 1979. Mg2+-dependent adenosine triphosphatase in isolated synaptic vesicles. Attribution of most of the enzymic activity to an actomyosin-like protein. J. Biochem. (Tokyo) 85:567–574.

    Google Scholar 

  23. Weil-Malherbe, H., andGreen, R. H. 1951. The catalytic effect of molybate on the hydrolysis of organic phosphate bonds. Biochem. J. 49:286–292.

    Google Scholar 

  24. Williams, R. J. P. 1982. Free manganese (II) and iron (II) cations can act as intracellular cell controls. FEBS Letters 140:3–10.

    PubMed  Google Scholar 

  25. Yamaguchi, I., Matsumura, F., andKodous, A. A. 1979. Inhibition of synaptic ATPase by Heptachlorepoxide in rat brain. Pestic Biochem. Physicol. 11:285–293.

    Google Scholar 

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Authors and Affiliations

  1. Toxicology Branch Hazard Evaluation Division (TS-769) Office of Pesticide Programs, Environmental Protection Agency, 20460, Washington, DC

    John D. Doherty

  2. Developmental and Metabolic Neurology Branch, National Institute of Neurological and Communicative Disorders and Stroke National Institutes of Health, 20205, Bethesda, Maryland

    Norman Salem Jr., Carl J. Lauter & Eberhard G. Trams

Authors
  1. John D. Doherty
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  2. Norman Salem Jr.
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  3. Carl J. Lauter
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  4. Eberhard G. Trams
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Doherty, J.D., Salem, N., Lauter, C.J. et al. Mn 2+-stimulatedATPase in rat brain. Neurochem Res 8, 493–500 (1983). https://doi.org/10.1007/BF00965105

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  • DOI: https://doi.org/10.1007/BF00965105

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